以小鼠骨髓间充质干细胞为饲养层分离培养胚胎干细胞的研究  

The Study of Using Bone Marrow Mesenchymal Stem Cells of Mouse as Feeder Layer Cells in Cultivation of Embryonic Stem Cells

作　　者：杨恕玲[1] 单守明[1] 何生虎[1] 

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《中国农学通报》2011年第1期370-373,共4页Chinese Agricultural Science Bulletin

基　　金：宁夏回族自治区科技兴农计划项目"奶牛疾病防控技术研究"

摘　　要：为了探求一种更简单有效地分离培养昆明小鼠胚胎干细胞的饲养层培养体系,实验中收集3.5天的昆明小鼠胚胎,接种于小鼠骨髓间充质干细胞的饲养层培养体系,来分离培养胚胎干细胞。结果在骨髓间充质干细胞的饲养层培养体系上,获得了典型的ES细胞样集落,经碱性磷酸酶染色呈阳性,且具有分化的潜能。实验结果表明,小鼠骨髓间充质干细胞可以作为饲养层来分离培养ES细胞,并且能保持ES细胞的体外未分化状态。In order to find the more simple method of preparition for the layer feeder cells, the blastocytes of 3.5 days embryo of Kunming mouse cultured on bone marrow mesenchymal stem cells were used to isolate and culture the ES cells. The colonies of ES cells were harvested to passage. The growth of stem cells identified was observed , and their biological vitality was determined by alkaline phosphatase activity assay and cell differentiation examination. The blastocytes of 3.5 days embryo from Km mouse could be cultured on the mouse bone marrow mesenchymal stem cells to develop into ES cells with maintenance of undifferentiated state in vitro .

关 键 词：骨髓间充质干细胞 胚胎干细胞 细胞培养 

分 类 号：Q343.6[生物学—遗传学]