几种植物白藜芦醇合酶基因保守序列的克隆与分析  

Cloning and Sequencing of DNA Encoding Conservative Regions of Resveratrol Synthase Genes in Leaves of Four Grape Plants

作　　者：林碧英[1] 钱昆[1] 肖宇[2] 胡鸢蕾[3] 

机构地区：[1]福建农林大学园艺学院,福州350002 [2]东北农业大学园艺学院,哈尔滨150030 [3]北京大学蛋白质工程与植物基因工程国家重点实验室,北京100871

出　　处：《中国农学通报》2011年第4期129-133,共5页Chinese Agricultural Science Bulletin

基　　金：福建省科技厅重点项目(2009N1003);福建省科技重大专项(2008NZ0002-1)

摘　　要：克隆并分析几种植物中白藜芦醇合酶基因(resveratrol synthase,RS)的片段。根据GenBank中已知的RS基因保守序列设计引物,采用PCR技术从葡萄科植物(蛇葡萄、三叶爬山虎、五叶爬山虎和云南爬山虎)、桑科植物(桑树)和豆科植物(花生)的幼嫩叶片中扩增出该基因的DNA片段。测序结果表明,片段长635bp,6个片段均不含内含子,分别编码211个氨基酸,均包含白藜芦醇合酶基因的特异片段和芪合酶(stilbene synthase,STS)家族特异性信号序列。利用DNAMAN软件构建系统进化树,结果显示这6种植物与GenBank中已发表的川鄂爬山虎的RS同源性高达94%。成功获得了6种植物的RS基因片段,并对其进行了同源性分析,为进一步克隆RS全长基因及分析其表达特性奠定基础。To clone and analysis the RS gene segment from several plants.PCR prime RS were designed according to the consensus sequence of some RS genes registered in GenBank.The results showed that 4 PCR products had 635 bp of neclotides without any introns in their DNA sequences and that they encoded 211amino acids.All of the fragments contained the conserved structure domains of RS gene,for example,specific for RS activity core and the family signature sequence for stilbene synthase.A further analysis of phylogenetic tree were constructed by the software of DNAMAN.The results showed that 6 plants had a close genetic relation with Parthenocissus henryana.The amino acids sequence homology was 94%.In this research,6 RSgene segments were obtained,and phylogenetic analysis was researched.It established basis on cloning thespan of RS and studying the characteristics of RS gene expression in the 6 plants.

关 键 词：白藜芦醇合酶基因 DNA 克隆 序列分析 

分 类 号：Q781[生物学—分子生物学]