从非变性聚丙烯酰胺凝胶中回收DNA小片段的两种方法比较  

Comparative Study on Two Methods of Recycling Short DNA Fragments from Nondenaturing Polyacrylamide Gel

作　　者：章蕾[1] 袁玲[1] 黄建安[1] 吴文亮[1] 陈东生[1] 刘仲华[1] 

机构地区：[1]湖南农业大学茶学教育部重点实验室/国家植物功能成分利用工程技术研究中心,长沙410128

出　　处：《中国农学通报》2011年第7期227-230,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"茶氨酸生物合成的基因转录调控研究"(3087157);湖南农业大学人才稳定基金"茶树重要功能基因的克隆与鉴定"(07WD22)

摘　　要：从聚丙烯酰胺凝胶中回收DNA片段是分子生物学中的常用技术,尤其针对于DNA小片段的回收。本试验对比了两种从非变性聚丙烯酰胺凝胶中回收DNA小片段的方法(煮沸法和水浴法),并用无水乙醇和3mol/L醋酸钠共沉淀纯化DNA小片段。采用聚丙烯酰胺凝胶电泳检测回收结果,通过紫外检测进行定量分析,结果显示:水浴法比煮沸法的回收率高。水浴法结合乙醇沉淀法还具有经济和操作简单的优点,可以广泛应用。Recycling DNA fragments from polyacrylamide gel is a common technique in molecular biology especially for recycling DNA short fragments.In this experiment,two methods （boiling method and water-bath method） were used to extract short DNA fragments which were co-precipitated with absolute ethanol and 3 mol/ L sodium acetate together.The results were compared by polyacrylamide gel electrophoresis.At last,short DNA fragments was analyzed by the method of ultraviolet absorption.It showed that the recovery rate of short DNA fragments in water-bath method was higher than boiling method.Water-bath method combined with ethanol precipitation for extracted short DNA fragments had many advantages,such as economical and convenient which should be advocated to use widespread.

关 键 词：非变性聚丙烯酰胺凝胶 DNA小片段 回收 纯化 

分 类 号：Q7[生物学—分子生物学]