脂质体转染巴马猪胎儿成纤维细胞条件的优化  

Optimization of Parameters of Exogene Transfection of Luchuan Pig Fetal Fibroblasts in Vitro Mediated by Liposome

作　　者：粟文俊[1] 蒋泓[2] 唐冬生[2] 洪亚辉[1] 

机构地区：[1]湖南农业大学,长沙410128 [2]佛山科学技术学院,广东佛山528000

出　　处：《中国农学通报》2011年第7期300-303,共4页Chinese Agricultural Science Bulletin

基　　金：粤港关键领域重点突破项目"高效转基因技术在转基因罗非鱼和猪的研究与应用"(2008A024200006)

摘　　要：研究旨在脂质体介导基因转染巴马猪胎儿成纤维细胞时获得较高转染效率,同时以GFP为报告基因探讨影响外缘基因转染效率的参数,如DNA和脂质体的用量、转染的细胞数量及细胞与DNA与脂质体复合物的孵育时间。结果表明,6μgDNA与15μL脂质体转染8h,可获得较满意的转染效率。This study was aimed to obtain higher efficiency in gene transfection into Bama Pig Fetal Fibroblasts and to study the role of green fluorescence protein （GFP） as a reporter system.We investigated the effect of parameter such as the dose of DNA and liposome number of cell transfected and exposure time of the cell to the DNA-liposome complexes.It was indicated that 6 μg DNA with 15 μL liposome resulted in the highest transfection efficiency after 8 h transfection.

关 键 词：脂质体 成纤维细胞 转染 巴马猪 绿色荧光蛋白 

分 类 号：S852.65[农业科学—基础兽医学]