淡水黑鲷5种同工酶的组织特异性研究  

Studies on 5 Isozymes in Different Tissues of Hephaestus Fuliginosus

作　　者：李娴[1] 王成武[1] 安丽[1] 张志山[1] 付佩胜[1] 

机构地区：[1]山东省淡水水产研究所山东省淡水水产遗传育种重点实验室,济南250117

出　　处：《中国农学通报》2011年第7期380-384,共5页Chinese Agricultural Science Bulletin

基　　金：山东省农业良种工程项目子项目"优质抗病速生鱼类良种选育"

摘　　要：运用聚丙烯酰胺垂直梯度凝胶电泳法对淡水黑鲷(Hephaestus fuliginosus)的6种组织(肝脏、心脏、脾脏、肾脏、肌肉和眼)的5种同工酶[酯酶(EST)、过氧化氢酶(CAT)、过氧化物酶(POD)、苹果酸脱氢酶(MDH)、乳酸脱氢酶(LDH)]进行了研究,并对其同工酶位点及酶谱进行了分析。结果表明,淡水黑鲷5种同工酶系统具有不同程度的组织特异性。EST检测到2个位点,每个位点都有2个等位基因。CAT检测到1个基因位点,存在2个等位基因。POD酶谱较复杂,有5个位点,Pod-2,3,4,5都检测到2个等位基因。MDH具有s-MDH和m-MDH两种类型,在各组织中均表达,s-MDH只形成一种二聚体,m-MDH仅在肝脏中有2条带,其余组织中为1条带。LDH在6种组织中都表达,而Ldh-5只在心脏中能检测到。Five isozymes （EST,CAT,POD,MDH,LDH） in six tissues （liver,heart,spleen,kidney,muscle,eye） of Hephaestus fuliginosus were studied by using vertical polyacrylamide gel gradient electrophoresis.Zymograms and loci of these 5 isozymes were analyzed.The results showed that these five isozymes presented tissue specificity to some degrees.Two sites were detected with EST and each site was encoded by 2 loci.Only one site was detected with CAT which included 2 loci.The zymogram of POD was relatively complicated,includeding 5 sites and 4 of which had alleles.Two types of MDH （m-MDH and s-MDH） were found in six tissues： s-MDH only showed one dimer;m-MDH had 2 bands in liver and 1 bands in other tissues.LDH was detected in six tissues and Ldh-5 was only found in heart.

关 键 词：淡水黑鲷 同工酶 组织特异性 

分 类 号：S917.4[农业科学—水产科学]