彩色马蹄莲2n配子育种技术初探  

Primary Studies on Breeding Techniques by 2n Gametes in colored Zantedeschia hybrid

作　　者：李洁筠[1] 吴红芝[1] 陈溪[1] 周涤[2] 

机构地区：[1]云南农业大学园林园艺学院,昆明650201 [2]北京市农林科学院蔬菜研究中心,北京100097

出　　处：《中国农学通报》2011年第8期108-113,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"2n配子对马蹄莲种质创新和PGI障碍克服的研究"(30960238);国家科技支撑项目"设施专用百合;彩色马蹄莲高效育种技术研究与新品种选育"(2009BADB8B04-2)

摘　　要：以彩色马蹄莲的栽培品种‘黑魔术’Blackmagic、‘火烈鸟’Flamingo为试验材料,研究彩色马蹄莲2n配子育种技术。选择刚从茎秆中抽出的幼小健壮小花,用0.15%的秋水仙素处理以诱导2n配子,将诱导的2n配子进行不同组合的杂交实验。结果表明:秋水仙素处理对彩色马蹄莲雄雌蕊发育有一定毒副作用,其中对雌蕊的损伤大于雄蕊;2n配子能在一定程度上克服杂交后代不育性;在相同条件下,经2n配子诱导处理的杂交种子与未处理的对照种子相比,种子萌发时间大幅度缩短(50天左右),叶片变薄、变大、叶色变浅,有些杂交植株表现出不同程度的畸变。通过对杂交后代的细胞学鉴定表明:处理配子杂交后代中获得染色体数目为48(2n=3x=48)的三倍体植株。The cultivars of coloredZantedeschia hybrid ： Black magic and Flamingo are used as experimental material.The young robust buds were selected to be treated with 0.15% colchicine to induce 2n gametes.The treated young flower buds between the two cultivars were crossed when pistil and stamena matured.The results showed that： colchicine had some poison impact on the development of the pistil and androecium,especially on that of pistil;2n gametes can overcome sterility of hybrids to a certain extent.Under the same conditions,the seed germination of hybrids derived from treated gametes was sped up greatly （about 50 d in advance） compared with that of control seed;the leaves were larger,thinner,and light color,and some plants performed malformation.The chromosome identification showed： triploids were achieved from the crossing of treated gametes with normal gametes and the chromosome number was 48 （2n=3x=48）.

关 键 词：彩色马蹄莲 2n配子 杂交育种 秋水仙素 

分 类 号：S682.2[农业科学—观赏园艺]