基于RAPD标记的芥蓝种质资源遗传多样性分析  

Genetic Diversity Analysis of Chinese Kale Germplasm Resources by RAPD Markers

作　　者：陈文文[1] 刘厚诚[1] 陈日远[1] 宋世威[1] 孙光闻[1] 

机构地区：[1]华南农业大学园艺学院,广州510642

出　　处：《中国农学通报》2011年第8期150-155,共6页Chinese Agricultural Science Bulletin

基　　金：国家科技部星火计划项目"蔬菜高效可持续生产技术体系建立与示范推广"(2008GA781001);广东省产学研重大项目"外向型设施蔬菜周年生产与产业化示范"(cgzhzd0809)

摘　　要：调查了44份芥蓝种质的植物学性状,并利用RAPD分子标记分析了其遗传多样性。结果从150条随机引物中筛选出20个引物,20个引物共扩增出177条谱带,其中多态谱带105条,平均每个引物扩增出8.9条谱带和5.3条多态性谱,多态性比率为59.32%。基于RAPD标记,利用NTSYS-pc2.11构建了聚类树状图谱,遗传距离为0.70时,44份芥蓝资源可聚成6大类群。芥蓝种质存在着一定的遗传多样性,但原产华南而且主要产区也在华南,遗传多样性要小于芸薹属其他蔬菜。The morphological characteristics of 44 Chinese kale accessions were investigated and the DNA polymorphism was analyzed by randomly amplified polymorphic DNA （RAPD） marker.20 primers selected from 150 primers amplified a total of 177 bands,of which 105 were polymorphic,with an average of 8.9 bands and 5.3 polymorphic fragments per primer.The polymorphic percentage was 59.32%.The dendrogram based on RAPD data was constructed by NTSYS-pc2.11.Cluster analysis showed that 44 Chinese kale accessions could be divided into six groups at the level of D=0.70.Because Chinese kale was from south China and the main area was in south China,the genetic diversity of Chinese kale was smaller than otherBrassica vegetables.

关 键 词：芥蓝 种质资源 RAPD 遗传多样性 

分 类 号：S635.9[农业科学—蔬菜学]