番茄组培再生体系优化研究  

Study on Tissue Culture and Regenerative System of Tomato

作　　者：马杰[1,2] 邱栋梁[1] 

机构地区：[1]福建农林大学,福州350002 [2]毕节地区农业科学研究所,贵州毕节551700

出　　处：《中国农学通报》2011年第8期185-189,共5页Chinese Agricultural Science Bulletin

基　　金：福建农林大学校长基金"类胡萝卜素生物合成基因转化番茄及其优化技术研究"(01020005)

摘　　要：对番茄再生体系中无菌苗的苗龄、不定芽诱导培养基和生根培养基的激素配比进行了优化。试验以‘浙杂905’、‘圣亚’和‘富丹’为材料。用番茄的茎段和子叶作为外植体,对外植体的出愈率、诱导率和不定芽生根情况进行差异比较。主要结果如下:选苗龄为4天的番茄幼苗。番茄子叶为外植体的最佳再生培养基配比为MSB5+3%蔗糖+0.6%琼脂+BA2.0mg/L+NAA0.1mg/L(pH5.8),‘浙杂905’、‘圣亚’和‘富丹’子叶的出愈率分别为97.00%、91.00%、86.00%,诱导率分别为64.67%、59%、53%。以番茄茎段为外植体的最佳再生培养基配比为MSB5+3%蔗糖+0.6%琼脂+BA2.0mg/L+NAA0.2mg/L(pH5.8),‘浙杂905’、‘圣亚’和‘富丹’茎段的出愈率分别为81.67%、76.00%、82.67%,诱导率分别为41.00%、48.67%、39.67%。不定芽生根培养基为:1/2MS+NAA0.1mg/L+IBA0.5mg/L。通过试验对比得出,番茄再生体系中选用无菌苗的最佳苗龄、不定芽诱导培养基和生根培养基的配比,子叶的再生能力要显著高于茎段。A series of experiments were made on how to filtrating tomato axenic seedling-age,hormone constitution on shooting and rooting medium in order to obtain the best way on regenerative system of tomato.‘Zheza 905’,‘Shengya’and‘Fudan’were used as the materials in the study.Tomato stem and cotyledon were cultured to study on the callus rate,induction rate and rooting rate.The results showed that the best seedling-age were 4 days.The best regeneration medium of tomato cotyledon explants included MSB5,3% sucrose,0.6% agar,BA 2.0 mg/L,NAA 0.1 mg/L with pH 5.8.The cotyledon callus rates of‘Zheza 905’,‘Shengya’and ‘Fudan’were 97.00%,91.00% and 86.00%,respectively.The induction rate were 64.67%,59% and 53%,respectively.While the medium for stem explants includes MSB5,3.0% sucrose,0.6% agar,2.0 mg/L BA,0.2 mg/L NAA with pH 5.8.The stem callus rate of‘Zheza 905’,‘Shengya’and‘Fudan’were 81.67%,76.00% and 82.67%,respectively.The induction rates were 41.00%,48.67% and 39.67%,respectively.The rooting medium included 1/2MS,IBA 0.5 mg/L,NAA 0.1 mg/L.Regeneration frequency from cotyledon was much higher than that of stems.

关 键 词：番茄 子叶 茎段 组织培养 

分 类 号：S641.2[农业科学—蔬菜学]