加拿大紫荆‘森林火焰’组培快繁技术研究  

Studies on the Techniques of Tissue Culture and Rapid Propagation for Cercis canadensis ‘Forest Pansy’

作　　者：李久亮[1,2] 苑兆和[1] 招雪晴[1] 尹燕雷[1] 朱孟涛 吴芳菲 

机构地区：[1]山东省果树研究所,山东泰安271000 [2]山东农业大学林学院,山东泰安271018 [3]临沂市河东区林业局,山东临沂276034

出　　处：《中国农学通报》2011年第6期64-67,共4页Chinese Agricultural Science Bulletin

基　　金：山东省农业良种产业化项目"优良园林绿化彩叶树种引进筛选"

摘　　要：以加拿大紫荆‘森林火焰’(Cercis canadensis‘Forest Pansy’)当年生嫩枝为试验材料,探讨不同培养基和植物生长调节剂如6-BA、IBA、NAA、TDZ对试管苗增殖以及生根的影响。结果表明:最适增殖培养基为DKW+TDZ0.03mg/L+PVP0.5mg/L;生根培养基为1/2MS+IBA25mg/L+AC0.6mg/L。此体系中增殖系数可达4.27,生根率73.3%。Young stem sections with buds ofCercis canadensis‘Forest Pansy’were used as explants for experiment. The effects of culture mediums and PGRs such as 6-BA,NAA,IBA,TDZ on proliferation and rooting of test-tube plantlets were discussed. The results showed that the medium suitable for proliferation was DKW＋TDZ 0.03 mg/L＋PVP 0.5 mg/L,the optimum rooting medium was 1/2 MS＋IBA 25 mg/L＋AC 0.6 mg/L. In this system,the highest propagation coefficient was 4.27 and the rooting rate was 73.3%.

关 键 词：加拿大紫荆'森林火焰’ 组织培养 增殖 生根 

分 类 号：S311[农业科学—作物栽培与耕作技术]