一种测定杀菌剂对灰霉病菌(Botrytis cinerea)毒力方法的改进  

Improvement of A Fungitoxicity Test of Fungicide to Botrytis cinerea

作　　者：陈治芳[1,2] 韩秀英[2] 王文桥[2] 赵建江[2] 张小风[2] 马志强[2] 刘颖超[1] 

机构地区：[1]河北农业大学植物保护学院农药系,河北保定071001 [2]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心,河北保定071000

出　　处：《中国农学通报》2011年第5期346-350,共5页Chinese Agricultural Science Bulletin

基　　金：农业公益性行业科研专项(200803074);"十一五"国家科技支撑计划项目(2006BAD08A03);河北省科技支撑计划项目(06220110D)

摘　　要：为探索一种灵敏度高、准确性高的筛选灰霉病防治药剂的试验方法,笔者通过在灰霉病菌(Botrytis cinerea)分生孢子悬浮液中加入1%酵母浸膏、2.5%葡萄糖和10%胡萝卜汁,接种离体黄瓜子叶,病叶率达100%,发病均匀度达98%以上,确立了一种重复性好、操作简便的测定杀菌剂对灰霉病菌毒力的新方法--黄瓜子叶喷雾法。以该方法、菌丝生长速率法和孢子萌发法分别测定比较了戊唑醇、代森锰锌和异菌脲对灰霉病菌的毒力,发现药剂浓度的对数值与其抑制率的几率值之间有很高的相关性,相关系数均大于0.95。该方法可用于灰霉病菌抗药性监测和杀菌剂敏感性测定。To seek for a high sensitive and high accuracy method to screen fungicides, in this research, a new test （cucumber cotyledons spray inoculation test） of Botrytis cinerea was established through inoculation on cucumber cotyledons with conidial suspension of B. cinerea amended with 1% yeast extract, 2.5% glucose and 10% carrot juice. The results showed that the test exhibits high disease incidence （100%） and uniform disease severity （98% ）, good repetition and simple operation. Inhibitory activity of tebuconazole, mancozeb and iprodione to Botrytis cinerea was tested and compared by cucumber cotyledons spray inoculation test, mycelial growth rate test and conidial germination test, it was found that a high correlation was shown between the logarithmic concentrations of fungicide and probability values of inhibition rate, the correlation coefficients were all above 0.95. This method could be used in fungicide sensitivity test and screening resistance level of fungicide against Botrytis cinerea.

关 键 词：灰霉病菌 接种 黄瓜子叶喷雾法 杀菌剂 室内毒力 

分 类 号：S131.2[农业科学—农业基础科学]