金铁锁总皂苷提取工艺研究  

Extraction Process of the Saponins in Psammosilene tunicoides W. C. WU et C. Y. Wu.

作　　者：吕小梨[1,2] 王华磊[1,2] 赵致[1,2] 周英[1,2] 罗春丽[1,2] 李金玲[1,2] 刘红昌[1,2] 徐绯[1,2] 

机构地区：[1]贵州大学中药材研究所,贵阳550025 [2]贵州省药用植物繁育与种植重点实验室,贵阳550025

出　　处：《中国农学通报》2011年第5期470-474,共5页Chinese Agricultural Science Bulletin

基　　金：贵州省科学技术基金项目"药用植物金铁锁种子质量形成机制和调控研究"(黔科合J字[2008]2071号)

摘　　要：采用正交设计,以齐墩果酸为对照品,采用5%香草醛冰醋酸-高氯酸显色剂,以总皂苷提取率为指标来确定金铁锁的最佳提取工艺。结果表明,最佳提取工艺如下:提取温度为70℃、乙醇浓度为80%、提取时间90min、料液比1:8,该方法成本低、结果稳定、重复性好。The orthogonal design was used to study the optimum process of extracting total saponins in Psammosilene tunicoides W.C. WU et C. Y. Wu. The oleanolic acid was selected as comparison product and 5% vanillin-acetic acid-HClO solution was selected as color-producing reagent. As an indicator, the extraction rate of total saponins was used to indicate the best extraction process. The optimum technological conditions were as follows： extraction temperature 70℃, ethanol concentration 80%, treat time 90 min, ratio of solid to liquor at 1：8. The optimized process had the advantages of low cost, stable and the excellent repeatability.

关 键 词：金铁锁 总皂苷 提取工艺 

分 类 号：S567[农业科学—中草药栽培]