肺岩宁方及其拆方对Lewis小鼠肿瘤肺转移与VEGF/KDR表达关系的影响  

Effects of "Feiyanning Decoction" and Its Decomposed Recipes on Expressions of VEGF/KDR mRNA and Proteins in Lewis Tumor Model Mice

作　　者：王中奇[1] 邓海滨[1] 张丽曼[1] 徐振晔[1] 

机构地区：[1]上海中医药大学附属龙华医院肿瘤科,上海200032

出　　处：《上海中医药大学学报》2011年第2期65-68,F0003,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市教委科研创新项目(08YZ61);上海市浦东新区中医领军型人才项目(PWZl2009-04)

摘　　要：目的:观察肺岩宁方及其拆方对C57小鼠Lewis肺癌肿瘤组织中血管内皮生长因子(vascularendothelial growth factor,VEGF)及其激酶功能区受体(kinase-domain insert containing receptor,KDR)mRNA与蛋白表达的影响。方法:建立C57小鼠Lewis肺癌模型,随机分为模型组、肺岩宁组、益气组、补肾组和抗癌组,每组10只。分别给予相应药物干预,均灌胃21 d,第22天处死。观察肺转移情况,取肿瘤组织,采用逆转录聚合酶链反应(RT-PCR)法和免疫组化SP法分别检测C57小鼠Lewis肺癌中VEGF和KDR mRNA及蛋白表达水平。结果:肺岩宁组与补肾组、抗癌组小鼠的肺转移率明显低于模型组与益气组(P<0.05)。C57小鼠Lewis肺癌组织中表达VEGF和KDR,但肺岩宁组与补肾组、抗癌组小鼠肿瘤组织中VEGF mRNA及蛋白的表达水平均明显低于模型组和益气组(P<0.05),肺岩宁方组和抗癌组小鼠肿瘤组织中KDR mR-NA及蛋白的表达水平均明显低于模型组和益气组(P<0.05)。结论:肺岩宁抑制C57小鼠Lewis肿瘤转移的机制可能与下调VEGF及其受体KDR mRNA和蛋白表达有关。Objective ： To observe the effects of ＂Feiyanning Decoction＂ and its decomposed recipes on expressions of vascular endothelial growth factor （VEGF） and kinase-domain insert containing receptor （KDR） mRNA and proteins in Lewis tumors of C57 mice. Methods： The Lewis lung cancer model of C57BL/6 mice were established and divided into model group （ A）, Feiyanning group （ B）, nourishing-qi group （ C）, tonifying-kidney group （D） and anti-cancer group （E） with 10 mice in each group. All the groups were treated by respective recipe for twenty-one days. The lung metastasis was observed and the expressions of VEGF and KDR mRNA and proteins were detected by reverse transcription-polymerase chain reaction （RT-PCR） method and immunohis- tochemical SP method respectively. Results： Compared with group A and C, the lung cancer metastasis rate decreased markedly in group B, D and E （P 〈0.05 ）. The expressions of VEGF and KDR exist in Lewis lung tumor, while the expressions of VEGF mRNA and protein in group B, D and E were lower than that in group A and C （ P 〈 0.05 ） , and the expressions of KDR mRNA and protein in group B and E were lower than that in group A and C （ P 〈 0.05 ）. Conclusion ： ＂ Feiyanning Decoction＂ can inhibit Lewis tumor metastasis in C57 mice and its mechanism may be related to down-regulating the expressions of VEGF and KDR mRNA and proteins.

关 键 词：肺岩宁方 LEWIS肺癌 肿瘤转移 血管内皮生长因子 激酶功能区受体 

分 类 号：R285.5[医药卫生—中药学]