清气凉营注射液中15个成分的HPLC/ESI/MS分析研究  

HPLC/ESI/MS analysis of the fifteen components in Qingqi Liangying Injection

作　　者：任爱农[1] 顾学红[2] 王大为[1] 

机构地区：[1]江苏省中医药研究院,江苏南京210028 [2]江苏大学药学院,江苏镇江212013

出　　处：《中成药》2011年第3期369-373,共5页Chinese Traditional Patent Medicine

基　　金：江苏省中医药局(HL07097)

摘　　要：目的:研究清气凉营注射液(大青叶,大黄,金银花,知母,野菊花和淡竹叶)的HPLC图谱中特征峰的化学成分进行初步判定,并确定各峰的药材归属。方法:色谱柱:Promosil C18柱(4.5 mm×250 mm,5μm);流动相:0.2%醋酸水溶液-甲醇,梯度洗脱;检测波长:325 nm,440 nm;流速:0.8 mL/min;电喷雾质谱(ESI-MS)分析,正、负离子一级扫描,扫描范围(m/z):150～650。结果:标示出了清气凉营注射液中的15个共有峰,采用HPLC/ESI/MS方法对清气凉营注射液的图谱中的主要色谱峰进行了初步结构判定,并说明了药材归属。结论:该方法稳定可靠,能够很好的用于清气凉营注射液中成分的分析。AIM：To establish a method for initially determining the structure of the active ingredients of Qingqi Liangying Injection（lsatidis folium,Rhei redix et rhizome,Lonicerae japonicae flos,Anemarrhenae rhizome,Chrysanthemi indici flos,Lophatheri herba） by HPLC/ESI/MS and to track down original plant.METHODS：Separa-tion was performed on a Promosil C18（4.5 mm×250 mm,5 μm） analytical column with mobile phase consisting of 0.2% acetic acid and methanol with gradient elute at the flow rate of 0.8 mL/min.The UV detection wavelength was set at 325 nm and 440 nm.An electrospray mass spectrometer was utilized for qualitative analysis and both pos-itive and negative ion scan mode were applied.The scanning zone ranged from 150 to 650.RESULTS：Fifteen common peaks of Qingqi Liangying Injection were separated,and with the help of UV and MS 12 compositions were elucidated.The peak identifications were determined at the same time.CONCLUSION：This method is stable and reliable.This method can be used for the analysis research of the components in Qingqi Liangying Injection.

关 键 词：清气凉营注射液 成分 HPLC/ESI/MS 分析 

分 类 号：R284.1[医药卫生—中药学]