p38MAPK活化在苦参碱诱导Raji细胞凋亡中作用的研究  

Effect of p38MAPK activating matrine-induced apoptosis Raji cells

作　　者：刘占术[1] 罗章琴[1] 张红宾[2] 陈建斌[2] 杨泽松[2] 黄曦[3] 黄宗干[2] 

机构地区：[1]重庆医科大学附属永川医院血液内科,重庆402160 [2]重庆医科大学附属第一医院血液内科,重庆400016 [3]重庆市合川区人民医院肿瘤血液中心,重庆401520

出　　处：《中成药》2011年第3期400-403,共4页Chinese Traditional Patent Medicine

基　　金：重庆市卫生局科研资助项目(08-2-035)

摘　　要：目的:探讨p38MAPK活化在中药苦参碱(Matrine,Mat)诱导人Burkitt,s淋巴瘤Raji细胞凋亡中的作用。方法:体外培养Raji细胞,分别用苦参碱(终浓度为0.4、0.8、1.6mg/mL)和在此基础上加入SB203580(p38 MAPK抑制剂)作用48h后,采用AnnexinⅤ–FITC/PI双染法检测细胞凋亡率,应用Western blot检测P-p38MAPK及Caspase-3蛋白表达量。结果:终浓度为0.4、0.8、1.6 mg/mL苦参碱对Raji细胞总凋亡率分别为(15.77±0.53)%、(27.88±1.52)%、(48.08±2.87)%、与加入SB203580比较(11.48±0.64)%、(19.34±0.91)%、(33.98±1.26)%具有统计学意义(P<0.05或P<0.01),与对照组(8.78±0.66)%比较也具有显著性差异(P<0.05或P<0.01);Western blot显示了P-p38MAPK和Caspase-3蛋白表达水平均随苦参碱浓度的提高而增加,加入SB203580后二者表达降低。结论:苦参碱可诱导Raji细胞凋亡,其凋亡可能与p38 MAPK的活化,进而直接或间接激活Caspase-3有关。AIM：To explore the effect and signaling pathway involved in apoptosis of human Burkitt＇s lympho-ma Raji cells induced by matrine through p38 MAPK activation.METHODS：Raji cells were cultured in vitro,the cells were treated by different final concentrations（0.4,0.8,1.6mg/mL） of matrine or combined with SB203580（p38 MAPK inhibitor） before matrine was added,then cocultured for 48 h,cell apoptosis rate was detected by An-nexinⅤ-FITC/PI double staining method,the P-p38 MAPK and caspase-3 protein expresssion of Raji cells were evaluated by Western blot.RESULTS：After cells were treated by matrine（0.4,0.8,1.6mg/mL）,the corresponding total apoptosis rate of treatment-groups was（15.77±0.53）%,（27.88±1.52）%,（48.08±2.87）% re-spectively,had statistical significance compared with adding SB203580 groups（11.48±0.64）%,（19.34±0.91）%,（33.98±1.26）%（P0.05 or P0.01）,respectively,also had statistical significance compared with control group（8.78±0.66）%（P0.05 or P0.01）.As the concentration of matrine gradually increased.theprotein expresssion levels of p-p38MAPK and caspase-3 also increased,and decreased after SB203580 was added.CONCLUSION：In view of the inducement of matrine to the apoptosis of Raji cells in vitro,we evaluate matrine from bean family that exhibits the activation of caspase-3 through directly and indirectly activating P-p38 MAPK.matrine can induce the apoptosis of Raji cells in vitro,its possible mechanism is that matrine activates caspase-3 directly or indirectly through p38 MAPK activation.

关 键 词：苦参碱 RAJI细胞 P-P38MAPK CASPASE-3 凋亡 

分 类 号：R966[医药卫生—药理学]