大承气颗粒中大黄酚在大鼠体内血药浓度的含量测定  

Assay of chrysophanol of Dachengqi Granule in rat serum

作　　者：刘俊红[1] 方步武[2] 伍孝先[1] 李棣华[1] 吴咸中[1] 

机构地区：[1]天津市中西医结合急腹症研究所,天津300100 [2]天津医科大学,天津300070

出　　处：《中成药》2011年第3期429-432,共4页Chinese Traditional Patent Medicine

基　　金：天津市高等学校科技发展基金资助项目(2001YY08);天津医科大学科学基金项目(2000KY31)

摘　　要：目的:建立中药复方制剂大承气颗粒(大黄、厚朴、枳实与芒硝)中大黄酚在大鼠体内血药浓度的测定方法。方法:用高氯酸和氯仿处理血标本。采用HPLC测定血中大黄酚含量,色谱柱为kromasil C18(250 mm×4.6 mm),流动相甲醇-水(含1%高氯酸)80:20,检测波长254 nm。结果:血药浓度在0.855～27.36μg/mL范围内线性关系良好,相关系数R=0.999 9,日内、日间相对标准偏差(RSD)分别为2.4%、3.3%;方法回收率94.8.%～101.2%。大承气颗粒中大黄的主要成分(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)被吸收入血,大黄酚达峰时间为1 h。结论:该方法准确、可靠,大黄酚可作为该复方制剂的一种质控指标及生物当量指标。AIM：To establish a method for determining chrysophanol content of Dachengqi Granule（Rhei radix et rhizome,Magnoliae officinalis cortex,Aurantii fructus immaturus and Natrii sulfas） in rat.METHODS：Rat serum was treated by perchloric acid and chloroform.HPLC was applied,with kromasil C18（250 mm×4.6 mm） column;MeOH-water（containing 1% HCLO4）（80：20） as mobile phase;detection wavelength was set at 254 nm.RESULTS：The intra-day and inter-day RSD was 2.4% and 3.3%;the linear range was between 0.855 and 27.36 μɡ/mL（r=0.999 6）;recovery rate was 94.8.%-101.2%.Aloeemodin,rhein,emodin,chrysophanoland physcion in Radix et Rhizoma Rhei were absorbed into blood of rats administered orally with Dachengqi Granule,and chrysophanol content reached the peak value in 1 h.CONCLUSION：The method established is accurateand reliable,chrysophanol content could be as controlling factor and bioequivalence for the compound preparationof Dachengqi Granule.

关 键 词：大承气颗粒 大黄酚 高效液相色谱法 血药浓度 

分 类 号：R284.1[医药卫生—中药学]