HPLC测定山楂叶中牡荆素-4″-O-葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的含量  

Determination of vitexin 4″-O-glucoside,vitexin rhamnoside,vitexin,rutin,hyperoside in Hawthorn leaves by HPLC

作　　者：赵彩云 王强[2] 

机构地区：[1]江苏省徐州药品检验所,江苏徐州221006 [2]中国药科大学,江苏南京210009

出　　处：《中成药》2011年第3期494-497,共4页Chinese Traditional Patent Medicine

摘　　要：目的:用高效液相法同时测定山楂叶中牡荆素-4〃-O-葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的含量。方法:采用HPLC法,色谱柱:Sepax HP C18柱(4.6 mm×250mm,5μm);流动相:A为乙腈-四氢呋喃(20:1),B为0.5%甲酸溶液,采用梯度洗脱;0～10min,A为14%～17%;10～26min,A为17%～18%;26～28min,A为18%～14%;检测波长:360nm,流速:1.0mL/min;柱温:32℃。结果:该方法加样回收率牡荆素-4″-O-葡萄糖苷:99.59%(RSD=0.50%),牡荆素鼠李糖苷:99.57%(RSD=0.96%),牡荆素:98.70%(RSD=1.10%),芦丁:99.35%(RSD=1.16%),金丝桃苷:99.28%(RSD=0.88%)。结论:该方法准确可靠,重现性好,可用于山楂叶的质量控制。AIM：To establish an HPLC method for simultaneously determining the level of vitexin 4″-O-gluco-side,vitexin rhamnoside,vitexin,rutin,and hyperoside in Hawthorn leaves.METHODS：The HPLC method was carried out on Sepax HP C18 column（4.6 mm×250 mm,5 μm）at the temperature of 32 ℃;the mobile phase consisted of A：CH3CN-THF（20：1）,B：0.5% formic acid gradient elution profile for A was in the range of 14%-17% within 0-10 min,17%-18% within 10-26 min and 18%-14% within 26-28 min,respectively.UV detection wavelength was set at 360nm,The flow rate was 1.0 mL/min.RESULTS：The average recoveries were 99.59% with RSD of 0.50% for vitexin 4″-O-glucoside;99.57% with RSD of 0.96% for vitexin rhamnoside;98.70% with RSD of 1.10% for vitexin;99.35% with RSD of 1.16% for rutin;99.28% with RSD of 0.88% for hyperoside,respectively.CONCLUSION：The method is simple with the adequate accuracy and selectivity.It can be used for the quality control of Hawthorn leaves.

关 键 词：山楂叶 牡荆素-4″-O-葡萄糖苷 牡荆素鼠李糖苷 牡荆素 芦丁 金丝桃苷 HPLC 

分 类 号：R284.1[医药卫生—中药学]