柱前衍生化HPLC法测定大鼠肝S9组分中川丁特罗对映体  

Determination of Trantinterol Enantiomers in Rat Liver S9 Fraction by Pre-column Derivatization HPLC

作　　者：唐婧[1] 江坤[1] 李坤杰[1] 李发美[1] 

机构地区：[1]沈阳药科大学,沈阳110016

出　　处：《中国药师》2011年第3期339-342,共4页China Pharmacist

摘　　要：目的:建立柱前衍生化HPLC法研究大鼠肝S9组分中川丁特罗对映体体外代谢的立体选择性。方法:以二-O-乙酰基-L-酒石酸酐(DATAAN)为衍生化试剂,采用反相高效液相色谱法拆分川丁特罗对映体,测定大鼠肝S9组分中川丁特罗对映体的浓度。结果:川丁特罗对映体达到基线分离,分离度为2.24,线性范围2.50～200μmol·L^(-1),平均回收率(S)-川丁特罗为89.3%,(R)-川丁特罗为91.3%。平均日内、日间RSD均小于15%。在大鼠肝S9组分中川丁特罗的代谢呈现立体选择性。结论:此法专属性强、准确可靠,可用于大鼠肝S9组分中川丁特罗代谢的立体选择性研究。Objective： To separate trantinterol enantiomers in rat liver S9 fraction by pre-column derivatization HPLC and investigate probable stereoseleetivity in the in vitro metabolism. Method ： The concentrations of trantinterol enantiomers in rat liver S9 fraction were determined by pre-column derivatization using diaeetyl-L-tartarie anhydride （DATAAN） as ehiral derivatization reagent followed by RP-HPLC assay. Result： A baseline separation of trantinterol enantiomers was achieved and the resolution was 2.24. The assay was linear within the range of 2. 50-200μmol· L^-1 for each enantiomer. The mean recovery was 89. 3% and 91.3% for （ R）-trantinterol and （S）-trantinterol, respectively. The intraand inter-day preeisions （RSDs） were both less than 15%. Conclusion： The method established for analyzing trantinterol enantiomers is accurate and specific. It has been successfully applied in the stereoseleetive metabolism study of trantinterol enantiomers in rat liver S9 fraction.

关 键 词：川丁特罗 对映体 肝S9组分 衍生化 

分 类 号：R917[医药卫生—药物分析学]