固相萃取-气相色谱法测定茶叶中多种有机磷农药  

Determination of Organophosphorus Pesticide Residues in Tea by Solid-phase Extraction Gas Chromatography

作　　者：游飞明 吴芳[2] 陆万平[2] 张庆[2] 张兰[2] 

机构地区：[1]福建省产品质量检验研究院,福建福州350002 [2]福州大学食品安全分析与检测教育部重点实验室,福建福州350002

出　　处：《分析测试技术与仪器》2011年第1期6-10,共5页Analysis and Testing Technology and Instruments

基　　金：国家自然科学基金(21075016);高等学校科技创新工程重大项目培育项目(708056);福建省自然科学基金项目(2009J01028);福建省质量技术监督局科技项目(FJQI2007004)资助

摘　　要：建立了固相萃取-气相色谱法同时测定茶叶中16种有机磷农药残留的方法.样品用丙酮超声提取后,经自制的N-丙基乙二胺(PSA)/活性炭固相萃取柱净化,以混合溶剂乙腈/甲苯(V(乙腈)∶V(甲苯)=1∶3)洗脱,采用气相色谱-氮磷检测器(GC-NPD)测定.方法的回收率在62.8%～109.1%之间,相对标准偏差小于14.7%1,6种有机磷农药的检出限在0.01～0.16 mg/kg之间.方法快速简便、经济实用。A method for the determination of 16 organophosphorus pesticide residues in tea by Gas Chromatography-Nitrogen Phosphorus Detector（GC-NPD） with ultrasonic extraction was established.Tea samples were extracted with 10 mL of acetone,and then cleaned by homemade PSA/activated carbon SPE cartridge and eluted with acetonitrile-toluene（1∶3,V/V）.16 organophosphorus pesticides were well separated and detected with high repeatability and precision.The recovery rate of the method was between 62.8% and 109.1%.The relative standard deviation was less than 14.7%.The limit of detection of 16 organophosphorus was between 0.01 mg/kg and 0.16 mg/kg.The method is reliable rapid and is,suitable for the analysis of pesticide residues in real samples.

关 键 词：固相萃取 气相色谱 茶叶 有机磷 农药残留 

分 类 号：O657.72[理学—分析化学]