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作 者:赵辉(综述)[1] Liwang Cui(审校) 杨照青(审校)[1]
机构地区:[1]昆明医学院寄生虫学教研室,云南昆明650031 [2]Department of Entomology, The Pennsylvania State University, UniversityPark, PA 16802, USA
出 处:《中国热带医学》2011年第4期501-503,共3页China Tropical Medicine
基 金:国家自然科学基金资助(No.30960050)
摘 要:间日疟原虫的研究由于体外培养技术的缺乏而进展迟缓。而体外连续性培养体系的建立由于间日疟原虫的本身特性及对侵入红细胞的特异性选择而受阻。近几年,研究用源自血色病患者血液和脐带血中的网织红细胞短期体外培养,可使间日疟原虫在体外生存达到一个月左右。目前,使用实验室源自脐带血造血干细胞分化的红系细胞能够不断的提供网织红细胞以及源于正常人肝组织的肝细胞株HC-04的新建立,完成了间日疟原虫体外连续性培养的目的。这里,我们着重介绍间日疟原虫的体外连续性培养技术。Research on Plasmodium vivax parasite has been slow due to the absence of a technique for continued culture of this parasite. The establishment of an in vitro culture system has been hampered by the requirement of reticulocytes for invasion of the parasite. In recent years,the use of reticulocyte-enriched blood from hemochromatotic patients and umbilical cord blood has allowed the extension of P. vivax in vitro culture to a month or so. To date, hematopoietic stem ceils from cord blood serve as a continuous source of reticulocytes. Together with the establishment of a hepatocyte line HC-04 for culturing P. vivax liver stages,this allows the completion of the in vitro culture of all P. vivax stages. Here we describe techniques for continuous in vitro cuhure of P. vivax.
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