Resonance Scattering Effect of Dopamine Product Particle and Its Application to Polyphenoloxidase Activity Assay  

作　　者：Jiang, Caina Ling, Shaoming Liang, Aihui Ouyang, Huixiang Wang, Pengfei Jiang, Zhiliang 

机构地区：[1]Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China

出　　处：《Chinese Journal of Chemistry》2011年第3期544-548,共5页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （Nos. 20865002, 20965002, 21075023）, the Natural Science Foundation ot Guangxi （Nos. 0991021 z, 0832260） and the Research Funds of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection （Guangxi Normal University）, Ministry of Education.

摘　　要：In pH 7.2 Na2HPO4-NaH2PO4 buffer solution, polyphenoloxidase （PPO） could catalyze the oxidization of do- pamine （DA） to form polymer particles in dark-red color, which exhibited a strong resonance scattering （RS） peak at 780 nm. In the chosen conditions, as the PPO activity increased, the RS intensity at 780 nm increased linearly. The increased RS intensity （△I780nm） was linear to the PPO activity in the range of 0.10--6.0 U·mL^- 1, with a regression equation of △I780 nm = 96.6C＋ 15.1, a relative coefficient of 0.9987 and a detection limit of 0.06 U·mL^-1 PPO. The proposed method was applied to detecting PPO activity in potato sample with satisfactory results.In pH 7.2 Na2HPO4-NaH2PO4 buffer solution, polyphenoloxidase （PPO） could catalyze the oxidization of do- pamine （DA） to form polymer particles in dark-red color, which exhibited a strong resonance scattering （RS） peak at 780 nm. In the chosen conditions, as the PPO activity increased, the RS intensity at 780 nm increased linearly. The increased RS intensity （△I780nm） was linear to the PPO activity in the range of 0.10--6.0 U·mL^- 1, with a regression equation of △I780 nm = 96.6C＋ 15.1, a relative coefficient of 0.9987 and a detection limit of 0.06 U·mL^-1 PPO. The proposed method was applied to detecting PPO activity in potato sample with satisfactory results.

关 键 词：POLYPHENOLOXIDASE DOPAMINE organic particle resonance scattering spectral assay 

分 类 号：O614.122[理学—无机化学] TS255.54[理学—化学]