Photosensitive Azopolymer Brushes via Atom Transfer Radical Polymerization for Protein Sensing  

作　　者：Zhang, Zhihong Wang, Yaoli Yan, Fufeng Peng, Donglai Ma, Zhi 

机构地区：[1]Henan Provincial Key Laboratory of Surface & Interface Science, Zhengzhou University of Light Industry, Zhengzhou, Henan 450002, China [2]Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China

出　　处：《Chinese Journal of Chemistry》2011年第1期153-158,共6页中国化学（英文版）

摘　　要：Novel photosensitive azopolymer brushes were synthesized via surface initiated atom transfer radical polymerization using initiator self-assembled on Au surface. The chemical structures of azobenzene derivatives were confirmed by Fourier transform infrared spectroscopy （FTIR） and nuclear magnetic resonance spectroscopy （NMR）. The surface morphology of azopolymers via atom transfer radical polymerization （ATRP） for different time was investigated by atomic force microscopy （AFM）. Additionally, the photoisomerization of azopolymer was measured by ultraviolet-visible spectroscopy （UV-Vis）, The results indicate that such azopolymers can undergo trans-cis-trans photoisomerization efficiently by photo-irradiation with UV light. Furthermore, this photoisomerization property could also induce the reversible adsorption of bovine serum albumin （BSA） adsorption on azopolymer brush surfaces. This adsorption kinetics of the reversible process can be measured by surface plasmon resonance （SPR） spectroscopy in situ. It suggests that the protein biochips could be regenerated safely by UV irradiation rather than by being rinsed with chemical reagents.Novel photosensitive azopolymer brushes were synthesized via surface initiated atom transfer radical polymerization using initiator self-assembled on Au surface. The chemical structures of azobenzene derivatives were confirmed by Fourier transform infrared spectroscopy （FTIR） and nuclear magnetic resonance spectroscopy （NMR）. The surface morphology of azopolymers via atom transfer radical polymerization （ATRP） for different time was investigated by atomic force microscopy （AFM）. Additionally, the photoisomerization of azopolymer was measured by ultraviolet-visible spectroscopy （UV-Vis）, The results indicate that such azopolymers can undergo trans-cis-trans photoisomerization efficiently by photo-irradiation with UV light. Furthermore, this photoisomerization property could also induce the reversible adsorption of bovine serum albumin （BSA） adsorption on azopolymer brush surfaces. This adsorption kinetics of the reversible process can be measured by surface plasmon resonance （SPR） spectroscopy in situ. It suggests that the protein biochips could be regenerated safely by UV irradiation rather than by being rinsed with chemical reagents.

关 键 词：AZOPOLYMER PHOTOISOMERIZATION atom transfer radical polymerization （ATRP） adsorption biosensor 

分 类 号：O631.5[理学—高分子化学] O631.24[理学—化学]