Synthesis, Characterization, DNA-binding Properties and DNA Cleavage of a New Ternary Copper（ll） Complex with Mixed-ligands of Tridentate Schiff Base and 1,10-Phenanth roline  

作　　者：Dong Jianfang Li Lianzhi Li Linwei Xu Tao Wang Daqi 

机构地区：[1]School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China [2]Department of Material Science, Shandong Polytechnic Technician College, Liaocheng, Shandong 252027, China

出　　处：《Chinese Journal of Chemistry》2011年第2期259-266,共8页中国化学（英文版）

基　　金：Project supported by the Natural Science Foundation of Shandong Province （No. Y2004B02）, the National Natural Science Foundation of China （No. 20543004） and the Special-purpose Construction Project Foundation for ＂Talshan Scholar＂ of Shandong Province.

摘　　要：A new copper（II） complex, [Cu（naph-leu）phen]CH3OH,0.5H20, in which naph-leu is the tridentate Schiff base ligand derived from the condensation of 2-hydroxy-l-naphthaldehyde and L-leucine, phen is phenanthroline, has been synthesized and characterized by elemental analyses, IR spectra and single crystal X-ray diffraction. The DNA-binding properties of this complex have been investigated by absorption spectra, fluorescence spectra and circular dichroism （CD） spectra, as well as viscosity measurement. Results show that this copper（II） complex binds to calf thymus DNA （CT-DNA） in an intercalative mode and its intrinsic binding constant Kb is 4.87 × 10^3 L.mol^-1. Furthermore, the DNA cleavage activity of this copper（II） complex has also been investigated by submarine gel electrophoresis. Interestingly, it was found that this complex can cleave the supercoiled plasmid pBR322 DNA to both nicked and linear forms.A new copper（II） complex, [Cu（naph-leu）phen]CH3OH,0.5H20, in which naph-leu is the tridentate Schiff base ligand derived from the condensation of 2-hydroxy-l-naphthaldehyde and L-leucine, phen is phenanthroline, has been synthesized and characterized by elemental analyses, IR spectra and single crystal X-ray diffraction. The DNA-binding properties of this complex have been investigated by absorption spectra, fluorescence spectra and circular dichroism （CD） spectra, as well as viscosity measurement. Results show that this copper（II） complex binds to calf thymus DNA （CT-DNA） in an intercalative mode and its intrinsic binding constant Kb is 4.87 × 10^3 L.mol^-1. Furthermore, the DNA cleavage activity of this copper（II） complex has also been investigated by submarine gel electrophoresis. Interestingly, it was found that this complex can cleave the supercoiled plasmid pBR322 DNA to both nicked and linear forms.

关 键 词：COPPER L-leucine Schiff base crystal structure DNA binding DNA cleavage 

分 类 号：O614.121[理学—无机化学] Q523[理学—化学]