破伤风类毒素对甲型副伤寒脱毒脂多糖的免疫增强作用  

The function of of S.paratyphoid A-tetanus toxoid's immunity enhancement to the detoxified lipopolysaccharide

作　　者：代长海[1] 柏丛[1] 黄菲[1] 魏春红[1] 吴梅[1] 

机构地区：[1]长春生物制品研究所,130062

出　　处：《中国实用医药》2011年第7期17-18,共2页China Practical Medicine

摘　　要：目的增强多糖抗原在婴幼儿中的免疫原性,提供有效的免疫保护。方法采用液体培养,收集菌体,脂多糖(LPS)提取,纯化脂多糖,活化脱毒多糖(O-SP)与破伤风类毒素(TT)共价结合。ELISA法检测O-SP及结合物的免疫效果。结果单纯甲型副伤寒脱毒脂多糖未能诱导小鼠产生抗体,而与TT结合后,具有良好的免疫原性,能产生体液免疫反应,诱导小鼠产生较高水平的抗脂多糖抗体和抗破伤风抗体。结论破伤风类毒类对甲型副伤寒脱毒脂多糖具有免疫增强作用。Objective To increase the immunognicity of detoxified lipopolysaccharide in infantile in order to provide stronger protection.Methods The detoxified lipopolysaccharide（LPS） antigen was obtained and purified from S.Paratyphi A strain which was multiplicated by submerged culture.The O-SP was covalent bond to the TT.The immunity effect was detected by ELISA.Results The IgG antibodies were not detected in the serum of mice immunized by detoxified lipopolysaccharide of S.Paratyphi A alone.The conjugate composed of detoxified LPS and tetanus toxoid elicited high anti-LPS IgG and anti-TT IgG level.Conclusion S.paratyphoid A-tetanus has immunity enhancement to the detoxified lipopolysaccharide.

关 键 词：甲型副伤寒脱毒脂多糖 破伤风类毒素 免疫原性 

分 类 号：R392[医药卫生—免疫学]