骨髓间充质干细胞治疗肌萎缩侧索硬化症的护理  

Nursing of the treatment of amyotrophie lateral sclerosis applying bone mesenchymal stem cells

作　　者：王翠林[1] 陈惠勤[1] 罗利英[1] 

机构地区：[1]广州市第十二人民医院血液科,510620

出　　处：《中华现代护理杂志》2011年第8期909-911,共3页Chinese Journal of Modern Nursing

摘　　要：目的观察骨髓间充质干细胞（MSC）治疗肌萎缩侧索硬化症（ALS）的临床效果，探讨护理方法。方法在无菌条件下采集患者骨髓液，密度梯度离心法分离，获取单个核细胞层，接种于培养瓶中，加入培养液，在5％二氧化碳细胞培养箱培养3d，微生物检测安全后静脉输注给患者。结果治疗后能延迟肌萎缩侧索硬化症患者的病情进展，有效8例，无效1例。结论MSC治疗肌萎缩侧索硬化症的临床效果有效。Objective To observe the therapeutic effects of bone marrow-derived mesenchymal stem cells （BMSCs） in the treatment of amyotrophie lateral sclerosis （ALS）. Methods To collect bone marrow in asepsis conditions. Mononuclear cells were collected by gradient centrifugation, cultured in growth medium and 5% carbon dioxide for 3 days. BMSCs were analyzed by flow cytometry with a FACScan （ Becton Dickinson）. All BMSCs were transplanted into patients intra venous. Results After BMSCs treatment, 8 patients with ALS had obvious clinical effects. 1 patients had no response to treatment. Conclusions BMSCs treatment for ALS has obvious clinical effect.

关 键 词：骨髓间充质干细胞 肌萎缩侧索硬化症 护理 

分 类 号：R473.74[医药卫生—护理学]