奇异变形杆菌分离株23S rRNA序列分析  

Sequence analysis of 23S rRNA genes of Proteus mirabilis

作　　者：王慧[1] 朱瑞良[1] 朱明华[1] 谭燕玲[1] 孙振红[1] 魏凯[1] 盛鹏程[1] 王新建[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2011年第4期476-479,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972183;30670114;30740077);山东省自然科学基金资助项目(Y2006D09)

摘　　要：通过PCR反应扩增7株鸡奇异变形杆菌和1株兔奇异变形杆菌的23SrRNA基因片段(1045bp),经克隆测序,用DNA Star分析软件将所获得的序列与GenBank中收录的奇异变形杆菌、普通变形杆菌以及其他相近属的23S rRNA基因序列进行同源性比较,并由此构建奇异变形杆菌系统进化发生树。结果表示,本实验室保存的7株鸡奇异变形杆菌核苷酸序列与GenBank中收录的奇异变形杆菌核苷酸序列同源性为99.4%～99.8%,与兔奇异变形杆菌核苷酸序列同源性为98.8%～99.3%,与普通变形杆菌的核苷酸序列同源性为95.4%～96.2%,而与其他相近属同源性只有92.9%～93.4%。结果表明,23S rRNA基因序列分析可以作为鉴定奇异变形杆菌的一种快速、简便的方法。To assess the evolution of Proteus mirabilis by 23S rRNA genes,To establish the sequence analysis of 23S rRNA of Proteus mirabilis was analyzed.The fragments（1 045 bp） of 23S rRNA genes of seven chicken Proteus mirabilis strains and one rabbit Proteus mirabilis strain were amplified by PCR and were sequenced.The sequences obtained in this paper were compared with the Proteus mirabilis 23S rRNA sequence reported in GenBank and other similar generas by the software of DNAStar analyses.The phylogenetic tree of Proteus mirabilis strains was established.Results showed the homologies between seven chicken Proteus mirabilis strains and the reported one strain in GenBank were 99.4%-99.8%,and were 98.8%-99.3% identical to the rabbit Proteus mirabilis strains,were 95.4%-96.2% identical to Proteus vulgaris,were 92.9%-93.4% identical to other similar generas.The result shown that 23S rRNA sequence analyses may be a reliable and rapid way for identification of Proteus mirabilis.

关 键 词：奇异变形杆菌 23S RRNA PCR 同源性 进化 分析 

分 类 号：S852.61[农业科学—基础兽医学]