禽肠炎沙门菌外膜蛋白OMPX基因的克隆及其在乳酸乳球菌中的表达  

Cloning and expression of Salmonella enteritidis OMPX gene in Lactococcus lactis

作　　者：陈金龙[1] 王希辉[1] 王婷婷[1] 胡敬东[1] 赵宏坤[1,2] 许瑞利 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]青岛农业大学食品科学与工程学院,山东青岛266109 [3]枣庄市市中区畜牧兽医局,山东枣庄277100

出　　处：《中国兽医学报》2011年第4期489-492,共4页Chinese Journal of Veterinary Science

基　　金：国家现代农业产业技术体系建设专项资金资助;山东省科技支撑计划资助项目(2007GG20009001);国家"十一五"科技支撑计划重大项目(2006BAK02A21)

摘　　要：以禽肠炎沙门菌基因组DNA为模板,采用PCR技术扩增得到外膜蛋白OMPX基因片段,并将其克隆到乳酸乳球菌表达载体pMG36e中,构建重组质粒pMG36e-OMPX。将重组质粒电转入乳酸乳球菌MG1614,得到重组基因工程乳酸乳球菌,在GM17培养基中培养12h后,经SDS-PAGE分析显示表达的蛋白约为16 000,与预期相符,经Western-blot检测表明,表达的蛋白具有良好的反应特异性。To clone and express the gene encoded the outer membrane protein X of Salmonella enteritidis.The PCR product was cloned into pMG36e vector,recombinant expression plasmid pMG36e-OMPX was constructed.Then the recombinants were transferred into the host strain MG1614,after cultured 12 hours in GM17 medium,SDS-PAGE showed a 16 000 protein was expressed and Western-blot confirmed the antigenicity against positive sera.The recombinant protein could be useful resource for development of genetic engineering Lactobacillus.

关 键 词：沙门菌 外膜蛋白 乳酸乳球菌 表达 

分 类 号：S852.61[农业科学—基础兽医学]