nephrin和ZO-1在糖尿病大鼠肾组织中的表达及其与足细胞数目的相关关系  

Expression of nephrin,ZO-1 in the kidney of diabetic rats and its correlation to the podocyte number

作　　者：殷玉红[1] 李玉兰[1] 王琴[1] 李英[2] 

机构地区：[1]河北省保定市第一中心医院,河北保定071000 [2]河北医科大学第三医院,河北石家庄050053

出　　处：《中国现代医学杂志》2011年第7期802-806,共5页China Journal of Modern Medicine

摘　　要：目的探讨糖尿病肾病发展过程中nephrin及ZO-1的表达变化及其与足细胞数目的相关关系。方法建立STZ诱导的糖尿病大鼠模型,应用免疫组织化学及RT-PCR方法观察在糖尿病肾病发展过程中nephrin、ZO-1蛋白及mRNA表达变化,以及应用WT-1来标记足细胞核,观察足细胞数目的变化。结果 8周时,糖尿病组nephrin和ZO-1染色强度较正常组明显降低(P<0.05),12周时变化更为明显(P<0.01),足细胞数目在两个时间点较正常组均明显减少。足细胞数目与nephrin及ZO-1表达呈正相关(r=0.93 P=0.0024;r=0.923,P=0.0086),而与尿蛋白呈负相关(r=-0.9508 P=0.0036)。结论 nephrin和ZO-1在糖尿病肾病发展过程中表达减低,并与足细胞数目呈正相关。nephrin和ZO-1的表达减低与足细胞数目减少以及尿蛋白的发生密切相关。【Objective】 To investigate the change of nephrin,ZO-1 expression and the correlation between the podocyte number and nephrin,ZO-1 expression.【Methods】 STZ-induced diabetic rats were established,immunohistochemical and RT-PCR were employed to study the change of nephrin,ZO-1 protein and mRNA.WT-1 was used to stain podocyte nucleu to investigate the change of podocyte number.【Results】 At week 8,the expression of nephrin,ZO-1 protein decreased dramatically（P 0.05）;At week 12,It decreased more obviously（P 0.01）.At two time points,the podocyte number decreased dramatically（P 0.05）.The podocyte number correlated positively with nephrin,ZO-1（r =0.93,P =0.0024,r =0.923 P =0.0086）.The podocyte number correlated negatively with urine protein（r =-0.95,P =0.0036）.【Conclusion】 The expression of nephrin,ZO-1 decrease in diabetic nephropathy and correlate positively with podocyte number,There is close relationship between the nephrin,ZO-1 expression,podocyte number and urine protein.

关 键 词：NEPHRIN ZO-1 足细胞数目 相关性 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]