ESI-MS法研究N-苯甲酰基-脱氢枞胺衍生物与血管紧张肽Ⅲ的相互作用  

Interaction Between N-Benzoyl-dehydroabietylamine Derivatives with Angiotensin Ⅲ by Electrospray Ionization Mass Spectrometry

作　　者：吕林[1] 邓慧敏[1] 林中祥[2] 

机构地区：[1]中山大学测试中心,广州510275 [2]南京林业大学化学工程学院,南京210037

出　　处：《高等学校化学学报》2011年第4期863-867,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:30771688;30871989)资助

摘　　要：采用电喷雾质谱法研究了4种新合成的N-苯甲酰基-脱氢枞胺衍生物(NBDD)———N-苯甲酰基-脱氢枞胺(NBD)、N-邻氯苯甲酰基-脱氢枞胺(NClBD)、N-苯甲酰基-脱氢枞胺-7-酮(NBDO)和N-邻氯苯甲酰基-脱氢枞胺-7-酮(NClBDO)与血管紧张肽Ⅲ(AngⅢ)的相互作用.结果表明,这4种化合物分别与AngⅢ以摩尔比1∶1,3∶1和5∶1混合时,均可形成化学结合计量比为1∶1和2∶1的非共价复合物,这些复合物主要电离为+2价离子[NBDD-AngⅢ+2H]2+和[(NBDD)2-AngⅢ+2H]2+.化学结合计量比为1∶1的NBDD-AngⅢ复合物+2价离子的稳定性顺序为:NBDO-AngⅢ>NClBDO-AngⅢ>NClBD-AngⅢ>NBD-AngⅢ;在化学结合计量比为2∶1的(NBDD)2-AngⅢ复合物中,2个NBDD分子与AngⅢ的结合形式为NBDD-AngⅢ-NBDD.The interactions between the novel N-benzoyl-dehydroabietylamine derivatives（NBDD）including N-benzoyl-dehydroabietylamine（NBD）,N-o-chlorobenzoyl-dehydroabietylamine（NClBD）,N-benzoyl-dehydroabietylamine-7-one（NBDO） and N-o-chlorobenzoyl-dehydroabietylamine-7-one（NClBDO） with angiotensin Ⅲ（AngⅢ） were investigated by electrospray ionization mass spectrometry（ESI-MS）.The results show that when the four derivatives respectively mixed with Ang Ⅲ in molar ratios of NBDD∶AngⅢ 1∶1,3∶1 and 5∶1,the non-covalent complexes with binding stoichiometries of 1∶1 NBDD-AngⅢ and 2∶1（NBDD）2-AngⅢ formed and the complexes mainly formed＋2 charged ions [NBDD-AngⅢ＋2H]2＋ and [（NBDD）2-AngⅢ＋2H]2＋.The stability order of the four kinds of complex ions of binding stoichiometries of 1∶1 [NBDD-AngⅢ＋2H]2＋ was NBDO-AngⅢNClBDO-AngⅢNClBD-AngⅢNBD-AngⅢ.And for the complexes of binding stoichiometries of 2∶1（NBDD）2-AngⅢ,the combination mode of two NBDD molecules with AngⅢ was NBDD-AngⅢ-NBDD.

关 键 词：电喷雾质谱 N-苯甲酰基-脱氢枞胺衍生物 血管紧张肽Ⅲ 相互作用 

分 类 号：O657.63[理学—分析化学]