芍甘多苷对四氯化碳亚急性肝损伤大鼠肝细胞线粒体保护作用  

Protective effects of Shaoganduogan on hepatocyte mitochondria in subacute liver injury rat induced by carbon tetrachloride

作　　者：宋军[1] 赵军宁[1] 王晓东[1] 戴瑛[1] 邓治文[1] 易进海[1] 

机构地区：[1]四川省中医药科学院药理毒理研究所,国家中医药管理局中药质量生物学评价重点研究室,中药品质评价与中药四川省重点实验室,四川成都610041

出　　处：《中国中药杂志》2011年第7期931-934,共4页China Journal of Chinese Materia Medica

基　　金：国家“十五”科技攻关计划项目(2001BA701A07-22);四川省重大科技招标项目(01SG003-33)

摘　　要：目的:探讨芍甘多苷对CCl4亚急性肝损伤大鼠血清转氨酶、肝脏病理及肝细胞线粒体保护作用。方法:采用CCl4诱发大鼠亚急性肝损伤,然后灌胃给予不同剂量的芍甘多苷治疗,观察药物对血清ALT,AST,肝脏组织病理,肝细胞线粒体超微结构和ATPase,MDA,SOD的影响。结果:芍甘多苷对CCl4诱发大鼠亚急性肝损伤升高的转氨酶有明显的降低作用,并使形态学上的肝细胞变性和坏死得到明显改善和恢复,能提高线粒体Na+-K+-ATPase,Ca2+-ATPase活性,降低线粒体MDA含量,升高SOD活性,改善线粒体超微病理变化,降低肝细胞线粒体截面面积、周长、等效直径、平均吸光度。结论:芍甘多苷有明显的肝脏保护作用,其机制与减轻线粒体损伤有关。Objective ： To study the protective effect of Shaoganduogan（SGDG） on serum transaminase, liver pathology and hepatocyte mitochondria in rat with subacute liver injury induced by carbon tetrachloride. Method ： Subacute liver injury of rats were induced by carbon tetrachloride, and cured by different doses of SGDG through intragastric administration. The activity of serum ALT,AST, liver pathology and ultrastructure, activity of ATPase, SOD and content of MDA of hepatocyte mitochondria were observed. Result ： SGDG can remarkably reduce the transaminase, alleviate the degeneration and necrosis of liver cells ,enhance activity of Na＋-K＋ATPase, Ca2＋ ATPase,SOD, reduce content of MDA of mitochondria, alleviate ultrastructure change of mitochondria, reduce section area, perimeter equivalent diameter and average optical density perimeter of liver cells. Conclusion ： SGDG has obvious effect of liver protection, the mechanisms are related with alleviating mitochondria injury.

关 键 词：芍甘多苷 肝脏保护 线粒体 

分 类 号：R285.5[医药卫生—中药学]