HPCE法同时测定保健品及中成药中的5种苷类成分  

Simultaneous determination of five kinds of glycosides in health care products and Chinese patent medicine by HPCE

作　　者：吴敏[1] 卢佳[1] 孙成均[2] 叶利民[1] 李章万[1] 钱广生[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川大学华西公共卫生学院,四川成都610041

出　　处：《华西药学杂志》2011年第2期165-167,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家"十一五"科技支撑计划课题资助(课题号:2006BAD27B02)

摘　　要：目的建立同时测定中成药及保健品中人参皂苷Rg1、Re、红景天苷、淫羊藿苷、腺苷5种苷类成分含量的毛细管电泳法。方法采用高效毛细管电泳紫外检测法,运行缓冲液为50 mmol·L-1硼砂-4 mmol·L-1NaCh(15%甲醇,pH10),分离电压30 kV,温度20℃;检测波长205 nm。结果人参皂苷Rg1、Re、红景天苷、淫羊藿苷、腺苷的线性范围分别为76.3～1220、120～1920、30～480、16.25～260、50.5～300μg·mL-1(r分别为0.9987、0.9997、0.9992、0.9989、0.9996),检测限分别为19.06、30.00、0.49、0.52、12.25μg·mL-1。结论 5种苷类成分可在20 min内得到良好的分离。OBJECTIVE To develop an HPCE-UV method for simultaneous determination of five kinds of glycosides in Chinese patent medicine and health care products.METHODS HPCE-UV was used with the buffer of 50 mmol·L-1Na2BO4·10H2O-4 mmol·L-1 NaCh（15% of methanol,pH10）.The applied voltage was 30 kV.The column temperature was at 20 ℃.The electropherograms was detected at 205 nm.RESULTS The linearity of ginsenoside Rg1,ginsenoside Re,rhodioloside,icariin and adenosine were obtained with the rang of 76.3-1220（r=0.9987）,120-1920（r=0.9997）,30-480（r=0.9992）,16.25-260（r=0.9989）,50.5-300（r=0.9996）μg·mL-1,respectively.The detection limits were 19.06,30.00,0.49,0.52,12.25 μg·mL-1,respectively.CONCLUSION This method has been successfully employed for the determination of five kinds of glycosides in 20 minutes.

关 键 词：高效毛细管电泳 中成药 保健品 苷类成分 

分 类 号：R917[医药卫生—药物分析学]