D2S388 D2S2232微卫星多态性与新疆维吾尔族吸烟者慢性阻塞性肺疾病易感性研究  

Correlation of microsatellite D2S388 and D2S2232 polymorphisms with susceptibility to chronic obstructive pulmonary disease among smokers of Uygur ethnic group in Xinjiang.

作　　者：杨永胜[1] 关键[1] 许西琳[1] 杨晓红 骆树新[1] 谷春玲 

机构地区：[1]石河子大学医学院第一附属医院呼吸科,新疆石河子832002 [2]新疆自治区人民医院呼吸科,乌鲁木齐830001

出　　处：《中国实用内科杂志》2011年第4期282-284,共3页Chinese Journal of Practical Internal Medicine

基　　金：国家自然科学基金(30960141)

摘　　要：目的研究D2S388、D2S2232微卫星多态性在新疆维吾尔族正常人群及慢性阻塞性肺疾病(COPD)患者中的分布并探讨D2S388、D2S2232微卫星多态性与新疆维吾尔族COPD易感性关系。方法提取石河子大学医学院第一附属医院呼吸科、新疆维吾尔自治区人民医院呼吸科、新疆阿克苏农一师医院呼吸科及新疆喀什地区第一人民医院呼吸科2009年3月至2010年9月住院的维吾尔族吸烟COPD患者118例和吸烟健康体检者110名(对照组)外周血基因组DNA。采用荧光标记的多重聚合酶链式反应扩增短串联重复序列(STR-PCR)结合毛细管电泳检测方法检测D2S388、D2S2232多态性。结果 D2S388位点共检测到9种等位基因和26种基因型,D2S2232位点共检测到9种等位基因和24种基因型。2个位点对照组基因型分布符合Hardy-Weinberg定律。D2S388位点254 bp等位基因频率在COPD组显著高于对照组(P<0.05);D2S2232位点204 bp等位基因频率在COPD组显著低于对照组(P<0.05)。结论 D2S388和D2S2232微卫星多态性可能与新疆维吾尔族吸烟者COPD易感性有关。Objective To investigate the distribution of D2S388 and D2S2232 length polymorphism of microsatellite loci in normal population and chronic obstructive pulmonary disease （COPD） smokers in Xinjiang Uygur ethnic group,and to study whether such polymorphism is associated with susceptibility to COPD. Methods Genornic DNA was extracted from peripheral blood of 118 COPD smokers and 110 healthy smokers （as controls） of Xinjiang Uygur ethnic group who were admitted or registered to Departments of Respiratory Medicine in First Affil- iated Hospital of Shihezi University School of Medicine, People＇s Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Aksu Agriculture No. 1 Division Hospital, and First People＇s Hospital of Kashgar Region Xinjiang, between March 2009 and September 2009. Polymorphisms of D2S388 and D2S2232 mierosatellite loci were determined by multiple short tandem repeat （STR） amplification using fluorescence labeling polymerase chain reaction （PCR） combined with capillary electrophoresis. Results Nine alleles and 26 genotypes were found in D2S388, and nine alleles and 24 genotypes in D2S2232. The distributions of both genotypes in healthy smokers were con- sistent with Hardy-Weinberg equilibrium. Compared with healthy controls, the COPD group showed significantly higher frequency of 254 bp D2S388 allele （ P 〈 0. 05 ） and lower frequency of 204 bp D2S2232 allele （ P 〈 0. 05）. Conclusion D2S388 and D2S2232 microsatellite polymorphisms may be associated with susceptibility to COPD in Xinjiang Uygur ethnic group.

关 键 词：慢性阻塞性肺疾病 微卫星多态性 遗传易感性 

分 类 号：R5[医药卫生—内科学]