玉米胚性愈伤组织继代及侵染条件的优化  

Optimization of Subculture Media and Infection Conditions of Embryogenic Callus of Maize

作　　者：钱丹丹[1] 焦丽[1] 邓川[1] 李晓薇[1] 张艳[1] 王庆钰[1] 李景文[1] 

机构地区：[1]吉林大学植物科学学院植物种质资源与利用实验室,吉林长春130062

出　　处：《作物杂志》2011年第2期71-74,共4页Crops

基　　金：吉林省科技厅重点项目(20080204);长春市科技计划项目(08GH10)

摘　　要：设计了含有不同2,4-D浓度的6种培养基用于玉米胚性愈伤组织的继代培养,对影响转化效率的侵染条件进行了研究,根据转化后所得的抗性愈伤组织数目及其分化率的评估结果表明,选用N6为基本培养基,添加1mg/L 2,4-D时,胚性愈伤组织继代长势较好,且在真空渗透的情况下,侵染5min,抗性愈伤组织数最多。经初步的PCR鉴定,利用优化的玉米遗传转化体系得到了转基因玉米植株。Six culture medias with different concentration of 2,4-D were designed and used for subculture of embryogenic callus of maize to investigate infection conditions that affect the efficiency of genetic transformation and to evaluate the number of antibiotic resistant callus and differentiation ratio after transformation.The results showed that N6 media supplemented with 2,4-D 1mg/L was suitable for subculture of embryogenic callus,If a vacuum pen-etration treatment and infection for 5min were also used,the most antibiotic resistant callus could be obtained.PCR analysis revealed that transgenic maize was obtained by using optimized genetic transformation system of maize.

关 键 词：玉米 愈伤组织 继代 农杆菌 转化 优化 

分 类 号：S513[农业科学—作物学]