透明质酸对关节软骨蛋白聚糖aggrecan的影响  

Effect of hyaluronic acid on articular cartilage proteoglycan aggrecan

作　　者：杜国辉[1] 屈爱存[2] 陈建英[2] 贺艳丽[1,2] 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东省生物药物研究院,山东济南250101

出　　处：《中国生化药物杂志》2011年第2期125-127,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究关节腔注射透明质酸对兔骨关节炎软骨基质的保护作用。方法关节滑液用软骨素酶ABC降解,丹磺酰肼柱前衍生。采用NH2色谱柱,流动相为乙腈-10 mmol/L醋酸溶液-10 mmol/L醋酸钠溶液(76∶16∶8),荧光检测器检测,激发波长350 nm,发射波长530 nm,流速为1 mL/min。结果透明质酸可减少兔关节滑液中C4S、C6S的含量。结论关节腔注射透明质酸可抑制兔软骨蛋白聚糖的降解或上调蛋白聚糖的合成,维持关节软骨正常的新陈代谢。Purpose To investigate the protective effect of intra-articular injection of hyaluronic acid on cartilage matrix in rabbit osteoarthritis. MethodsJoint synovial fluid was degraded by chondroitinase ABC,and then derived with dansylhydrazine.The derivatized sample was separated on NH2 column.The mobile phase of acetonitrile-acetic acid-sodium acetate（76：16：8） was used.Detected with fluorescence detector,the excitation wavelength（λex） and emission wavelength（λem） were 350 and 530 nm,respectively.The flow rate was 1.0 mL/min.Results Hyaluronic acid could decrease the contents of C4S and C6S in rabbit joint synovial fluid.Conclusion The intra-articular injection of hyaluronic acid can reduce the degradation of aggrecan and/or enhance the synthesis of aggrecan in the joint tissues of rabbit osteoarthritis,and maintain the normal metabolism of articular cartilage.

关 键 词：透明质酸 硫酸软骨素 蛋白聚糖 

分 类 号：R963[医药卫生—微生物与生化药学]