冠心合剂对家兔颈动脉球囊损伤后Ⅰ型胶原表达的影响  

Effect of Guanxin Decoction on Expression of Type-Ⅰ collagen in Rabbits after Carotid Artery Balloon Injury

作　　者：祝光礼[1] 周凡[1] 方伟[1] 魏丽萍[1] 刘昭[1] 

机构地区：[1]浙江中医药大学附属广兴医院心内科,浙江杭州310007

出　　处：《中华中医药学刊》2011年第4期689-691,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：杭州市卫生局科研项目(2007B0074)

摘　　要：目的:观察冠心合剂对兔颈动脉经皮冠状动脉腔内成形术(PTCA)损伤后细胞外基质Ⅰ型胶原蛋白表达的影响。方法:运用球囊原位扩张、拉伤致兔颈总动脉损伤建立PTCA术后内皮损伤模型。从术前3天开始各治疗组予相应剂量冠心合剂灌胃给药,分别于2、4、6周处死动物,免疫组织化学法检测Ⅰ型胶原的表达。结果:各中药治疗组内膜胶原密度较模型组降低,差异有统计学意义(P<0.05);冠心合剂大剂量组6周的内膜胶原密度表达最少。结论:冠心合剂可保护兔颈动脉球囊损伤后内膜,其机制可能与减少血管壁胶原含量有关。Objective：To observe the effect of Guanxin decoction on extracellular matrix collagen Ⅰexpression after percutaneous transluminal coronary angioplasty（PTCA） of rabbits.Methods：Endothelial injury models were established by using in-situ balloon expansion and strain-induced injury of rabbit carotid artery.The Guanxin decoction groups were fed correspondingly does 3 days before the operation.All the animals were sacrificed at the end of the 2ed,4th and 6th weeks respectively and operated carotid arteries were obtained.Immunohistochemical Strept Avidin-Biotin complex（SABC） staining were used for observing arterial morphology and intimal collagen density.Results：The Guanxin decoction groups have lower levels of intimal hyperplasia and intimal collagen（P0.05）.The large-does Guanxin decoction group has the lowest densities of intimal collagen at the end of the 6th week.Conclusion：Guanxin decoction could decreased the neointimal hyperplasia after PTCA,the mechanism may relate to inhibiting the expressions of collagen typeⅠin the injured artery.

关 键 词：冠心合剂 球囊损伤 Ⅰ型胶原 

分 类 号：R540[医药卫生—心血管疾病]