未分化骨髓基质干细胞体外抑制淋巴细胞增殖反应的初步研究  

Undifferentiated bone marrow stromal cells suppress lymphocyte proliferation in vitro

作　　者：沈海刚[1] 李晔[1] 刘波[2] 

机构地区：[1]杭州市中医院血管外科,浙江杭州310007 [2]浙江省中医院,浙江杭州310006

出　　处：《中华中医药学刊》2011年第4期783-785,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y2080180);杭州市卫生局重点科技项目(2003Z006)

摘　　要：目的:研究犬未分化骨髓基质干细胞(bone marrow stromal cells,BMSCs)体外抑制淋巴细胞增殖的作用。方法:从犬骨髓组织中分离获取骨髓基质干细胞,体外培养至第二代,1×105细胞/孔BMSCs刺激异体淋巴细胞,观察淋巴细胞增殖情况。以植物血凝素(PHA,2ug/mL)刺激淋巴细胞后,分别以1×102～5细胞/孔数量的异体BMSCs加入反应体系中,观察淋巴细胞增殖抑制情况。分别以1×102～5细胞/孔数量的"第三者"BMSCs加入双向混合淋巴细胞反应体系中,观察淋巴细胞增殖抑制情况。通透性隔离腔培养的BMSCs加入双向混合淋巴细胞反应体系中,观察淋巴细胞增殖抑制情况。结果:BMSCs刺激同种异体淋巴细胞体外增殖作用不明显;BM-SCs可明显抑制PHA刺激的淋巴细胞增殖(P<0.05);"第三者"BMSCs可明显抑制双向混合淋巴细胞反应(P<0.05),抑制作用呈BMSCs细胞数量依赖性;隔离腔培养的BMSCs也可明显抑制双向混合淋巴细胞反应(P<0.05)。结论:犬未分化BMSCs在体外具有抑制淋巴细胞增殖的作用,具有一定的调节淋巴细胞反应的能力。Objective：To study the effect of canine undifferentiated BMSCs surpressing lymphocytes proliferation.Methods：Canine BMSCs was isolated from bone marrow tissue and culture expanded.Peripheral blood lymphocytes（PBL） were cultured with allogeneic BMSCs at dose of 1×105.Canine BMSCs at a dose 1×102,1×103,1×104,1×105 were added to lymphocyte reactions induced by PHA and were added to the ongoing two-way mixed lymphocyte reactions（MLR） inspectively.BMSCs cultured in transwell chamber were added to two-way mixed lymphocyte reactions.Value of CPM（count per minute）was calculated with luminenscence counter.Results：No lymphocyte response was induced by allogeneic BMSCs as stimulators.Canine BMSCs could suppress lymphocyte proliferation induced by PHA and could elicit inhibit function of mixed lymphocyte cultures in a dose dependent way.BMSCs cultured in transwell chamber could also suppress two-way mixed lymphocyte reactions.Conclusion：Undifferentiated BMSCs could suppress lymphocyte proliferative responses induced by cellular or nonspecific mitogenic stimuli and could modulate immune responses in vitro.

关 键 词：未分化骨髓基质干细胞 淋巴细胞 抑制 

分 类 号：R331[医药卫生—人体生理学]