果蝇生殖包囊固定染色后的荧光特性  

Fluorescent Features of Drosophila Germline Cysts after Commonly Fixation and Staining

作　　者：王冰[1] 王晶[1] 李纪同[1] 杨磊[1] 张永忠[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《安徽农业科学》2011年第7期3785-3787,3797,共4页Journal of Anhui Agricultural Sciences

基　　金：山东省自然科学基金项目(Y2007D06)

摘　　要：[目的]探索果蝇卵巢及生殖包囊经过常规固定和染色后的荧光特性,为荧光组织学的发展提供第一手资料。[方法]以黑腹果蝇卵巢为试材,用Bouin氏液固定,石蜡切片后分别用HE、苏木精、伊红染色,在明场(白光)、绿色、蓝色和紫外激发光下观察、拍照。[结果]3种方法染色后,再经不同激发光激发,生殖细胞和体细胞可发出不同颜色的荧光,细胞中脂类、核酸及蛋白质也可分别发出各自特异性的荧光。[结论]常规染色剂可赋予生殖细胞和体细胞各自不同的荧光特性,也可赋予细胞内不同物质成分各自不同的荧光特性。[Objective] In order to provide first hand information for the development of fluorescent histology,the fluorescent features of Drosophila germline cysts after commonly fixation and staining were studied.[Method] Drosophila ovaries were used as experimental materials.After fixation in Bourn＇s solution,the ovaries were sectioned and stained with HE,Haematoxylin and eosin,respectively.The specimens were observed and photographed under fluorescent microscope in bright field,and fluorescent fields after excitation with green,blue and UV light,respectively.[Result] Speciments stained by the three commonly used staining methods showed different fluorescent features in their different cell typies（such as germ cells and somatic cells） and cellular components（such as lipids,nucleic acids and proteins） after excited with grean,blue and UV excitation light source,respectively.[Conclusion] The commonly used staining techniques could endow different cell types and cellular components different fluorescent features.

关 键 词：果蝇 生殖包囊 苏木精 伊红 荧光 

分 类 号：Q96[生物学—昆虫学]