山羊核移植胚胎干细胞的分离培养  

Isolated Culture of Nuclear Transplantation Embryonic Stem Cells of Goat

作　　者：葛秀国[1] 李吉霞[1] 窦忠英[2] 

机构地区：[1]天津农学院动物科学系,天津300384 [2]西北农林科技大学陕西省干细胞工程技术研究中心,陕西杨凌712100

出　　处：《安徽农业科学》2011年第7期4012-4014,共3页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究与发展项目(2005AA219050)

摘　　要：比较了不同发育阶段山羊核移植胚胎分离培养胚胎干细胞(NT-ESCs)的情况。结果表明,各阶段山羊核移植胚胎均可分离出NT-ESCs,其中孵化胚的NT-ESCs克隆形成率最高(38%),与囊胚(26%)差异显著,与桑葚胚(18%)差异极显著。所分离的NT-ESCs细胞形态与正常ESCs相似,均具有碱性磷酸酶活性,可表达多能性基因Oct-4和表面标志抗原SSEA-1。The isolated cultures of nuclear transplantation embryonic stem cells（NT-ESCs） from nuclear transplantation embryos of goats in different developmental stages were compared.The results showed that NT-ESCs could be isolated from nuclear transplantation embryos of goats in all developmental stages,among which the cloning efficiency of NT-ESCs from hatching embryos was highest,being 38%;it had significant difference from that of blastula,which was 26% and it had extremely significant difference from that of embryotic sphere,which was 18%.The shape of isolated NT-ESCs was similar to that of normal ESCs,both of them had alkaline phosphatase activities and could express pluripotency gene Oct-4 and surface marker antigen SSEA-1.

关 键 词：山羊 核移植 胚胎干细胞 

分 类 号：S826.94[农业科学—畜牧学]