基于丝网印刷碳电极的微囊藻毒素-(亮氨酸-精氨酸)的电流型免疫传感器  

An Amperometric Immunosensor for Microcystin-(Leucine-Arginine) Based on Screen-Printed Carbon Electrode

作　　者：陈向强[1] 何苗[1] 施汉昌[1] 蔡强[1,2] 

机构地区：[1]清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京100084 [2]浙江清华长三角研究院生态环境研究所,嘉兴314050

出　　处：《分析化学》2011年第4期443-448,共6页Chinese Journal of Analytical Chemistry

基　　金：国家高新技术发展计划(Nos.2007AA06Z419;2009AA06A417)资助项目

摘　　要：将微囊藻毒素-（亮氨酸-精氨酸）-鸡卵白蛋白（Microcystin-（leucine-arginine）-ovalbumin,MCLR-OVA）固定在锇联吡啶聚（4-乙烯基吡啶）聚合物修饰的丝网印刷碳电极表面,制备了一种检测MCLR的电流型免疫传感器。该传感器基于间接竞争免疫分析模式,以辣根过氧化物酶偶联的羊抗鼠免疫球蛋白抗体为标记物。其电流信号响应与MCLR的浓度在0.43~10.72 mg/L范围内呈负线性相关,检出限为0.17 mg/L。3种实际水样的平均添加回收率为83%~121%,相对标准偏差为3.6%~7.6%。本方法为MCLR的环境监测提供了科学依据。An amperometric immunosensor for the detection of microcystin-（leucine-arginine）（MCLR） was prepared by immobilizing an MCLR-ovalbumin（MCLR-OVA） conjugate to an osmium bipyridyl complex-attached poly（4-vinylpyridine） membrane modified onto a screen-printed carbon electrode.The immunosensor was based on the indirect competitive immunoassay format with the labeled agent of horseradish peroxidase-conjugated goat anti-mouse immunoglobulin-G antibody.The current response decreased proportionally with the concentration of MCLR in the range of 0.43-10.72 mg/L with a detection limit of 0.17 mg/L.The mean recoveries of standard additions in three real water samples were in the range of 83% to 121%,and the RSD were in the range of 3.6% to 7.6%.Thus,the proposed method is a promising technique for the detection of MCLR in environmental monitoring.

关 键 词：免疫传感器 微囊藻毒素-(精氨酸-亮氨酸) 锇氧化还原聚合物 丝网印刷碳电极 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]