RP-HPLC同时测定槐角丸中4种有效成分的含量  

Simultaneous Determination of Four Effective Components in Huaijiao Pills by RP-HPLC

作　　者：魏晓亮[1] 王冬梅[1] 张瑜[2] 王鸽[1] 薛秋霞[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]沈阳药科大学中药学院,沈阳110016

出　　处：《中国药学杂志》2011年第7期549-552,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立同时测定槐角丸中槐角苷、黄芩苷、染料木素和汉黄芩素4种有效成分含量的HPLC方法。方法采用Dia-monsil C18柱（4.6 mm×200 mm,5μm）进行分离;流动相为乙腈-0.05%磷酸溶液,梯度洗脱（0~10 min,25%~37%乙腈;10~25 min,37%~60%乙腈）;检测波长260 nm;柱温30℃;流速1.0 mL.min-1;进样量10μL。结果槐角苷、黄芩苷、染料木素和汉黄芩素的质量浓度与峰面积分别在11.6~116μg.mL-1（r=0.999 6,n=6）、33.5~335μg.mL-1（r=0.999 8,n=6）、0.46~4.63μg.mL-1（r=0.999 8,n=6）和1.54~15.4μg.mL-1（r=0.999 7,n=6）内呈良好的线性关系;平均加样回收率依次为99.3%、99.6%、99.6%和99.0%。RSD为1.1%、1.1%、1.4%和0.8%。结论该方法快速、简便、重复性好,适合于同时测定槐角丸中槐角苷、黄芩苷、染料木素和汉黄芩素的含量。OBJECTIVE To develop a RP-HPLC method for the determination of sophoricosid,baicalin,genistein and wogonin in Huaijiao pills.METHODS A Diamonsil C18 column（4.6 mm×200 mm,5μm） was used at 30 ℃.The mobile phase consisted of acetonitrile-0.05% phosphoric acid aqueous under gradient elution（0-10 min,25%-37% acetonitrile;10-25 min,37%-60% acetonitrile）.The flow rate was set at 1.0 mL·min-1.The detection wavelength was 260 nm.RESULTS The linearities ranged in 11.6-116 μg·mL-1 for sophoricosid（r=0 999 6,n=6）,33.5-335 μg·mL-1 for baicalin（r=0.999 8,n=6）,0.46-4.63 μg·mL-1 for genistein（r=0.999 8,n=6）,1.54-15.4 μg·mL-1 for wogonin（r=0.999 7,n=6） respectively.Recoveries were 99.3% with RSD 1.1% for sophoricosid,99.6% with RSD 1.1% for baicalin,99.6% with RSD 1.4% for genistein,99.0% with RSD 0.8% for wogonin.CONCLUSION The method is quick,simple and repeatable for simultaneous determination of sophoricosid,baicalin,genistein and wogonin in Huaijiao pills.

关 键 词：槐角丸 槐角苷 黄芩苷 染料木素 汉黄芩素 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]