肺结核患者外周血记忆性T细胞及B细胞亚群的检测分析  

Analysis of memory T cell and B cell subsets in the peripheral blood of patients with pulmonary tuberculosis

作　　者：朱宇珍[1] 郑学宝[1] 张明霞[2] 陈心春[2] 廖明凤[2] 邓群益[2] 

机构地区：[1]广东医学院中医教研室,广东广州524023 [2]深圳市第三人民医院,广东深圳518020

出　　处：《临床肺科杂志》2011年第5期667-669,共3页Journal of Clinical Pulmonary Medicine

基　　金：国家十一五专项基金(2008ZX10003-012);国家自然基金(编号:30972634)

摘　　要：目的探讨肺结核患者外周血记忆性T及B细胞亚群的表达水平。方法采用流式细胞仪检测肺结核患者(TB)、潜伏感染者(LTBI)、健康人(HD)外周血记忆性T、B细胞亚群水平及B细胞表达水平;利用Elispot技术检测外周血单个核细胞(PBMC)结核菌抗原特异性IFN-r分泌水平。结果流式结果显示,TB及LTBI的CD4+及CD8+中心记忆性T细胞(Tcm)显著增多,以CD4+尤为显著;相反,效应记忆性T细胞(Tem)减少。TB记忆性B细胞均低于HD和LTBI(P<0.01);而在HD与LTBI及TB这三组CD19+细胞占淋巴细胞比例中都有明显差异(P<0.01;P<0.001)。CD4+/CD8+Tcm与IFN-γELISPOT结果成正相关。结论肺结核患者外周血中存在着记忆性T及B细胞亚群的紊乱,中心记忆性T细胞增多,效应记忆性T细胞减少,记忆性T、B细胞的数量及免疫状态与结核菌感染的不同转归有关,记忆性T、B细胞在结核病发病机制中发挥着重要作用。Objective To study the expression of memory T cell and B cell subsets of peripheral blood in patients with pulmonary tuberculosis. Methods The memory T cell and B cell subsets of peripheral blood were analyzing with flow cytomety ; Antigen specific-IFN-γ response were determined in PBMCs by using in-house Elispot. Results CD4＋ and CD8＋ central memory T cells in TB and LTBI were much higher ,especially CD4＋ . On the contrary, effective memory T cells were reduced. Memory B ceils in TB were decreased compared with HD and LTBI. （ P 〈0. 01 ,P 〈0. 001 ）. The proportion of CD4 ,CD8＋ central memory T ceils is signi？ cantly correlated with the released levels of specific IFN-γ. Conclusion Memory T and B cell subsets were confused in peripheral blood of patients with pulmonary tuberculosis, Central memory T cell increased, while effort memory T cell decreased ; the population of memory T/B cells and immu- nization were associated with the result of Mycobacterium tuberculosis infection. Memory T and B cells play an important role in the tuberculosis mechanism.

关 键 词：记忆性T细胞 记忆性B细胞 B细胞 免疫记忆 

分 类 号：R521[医药卫生—内科学]