酶联免疫斑点试验检测技术(Elispot)在诊断结核性脑膜炎及Ⅲ型肺结核中的应用价值  

Implication of Elispot assay for diagnosis of tuberculous meningoencephalitis and Ⅲ type pulmonary turberculosis

作　　者：张培泽[1] 叶涛生[1] 邓永聪[1] 蔡雄茂[1] 陈涛[1] 张明霞[2] 

机构地区：[1]深圳市第三人民医院结核病科,广东深圳518020 [2]深圳市第三人民医院肝病研究所,广东深圳518020

出　　处：《临床肺科杂志》2011年第5期721-722,共2页Journal of Clinical Pulmonary Medicine

摘　　要：目的评价酶联免疫斑点技术(Elispot)检测外周血结核杆菌抗原特异性r干扰素(IFN-r)水平在结核脑膜脑炎及Ⅲ型肺结核患者诊断中的应用价值。方法利用Elispot技术分别对383例结核病人(包括73例结核性脑膜炎患者、310例Ⅲ型肺结核患者)、97例健康人外周血结核杆菌抗原特异性r干扰素(IFN-r)水平进行定量检测。结果 Elispot技术在结核病中诊断敏感性为81.46%,特异性为85.57%;结核脑膜脑炎组Elispot阳性率为61.64%;Ⅲ型肺结核患者组Elispot阳性率为86.13%;健康对照组Elispot阳性率为10.31%;各组间比较差异具有统计学意义(P<0.05)。结论 Elispot技术在结核病诊断中具有良好的辅助诊断价值,在Ⅲ型肺结核中的诊断价值优于结核性脑膜脑炎。Objective To investigate mycobactefium tuberculosis antigen specific IFN-r response（Elispot） in patients with tuberculous meningoencephafitis or Ⅲ type pulmonary turberculosis. Methods 383 cases of tuberculosis patients （ including 73 cases of tuber- culous meningoencephalitis and 310 cases of Ⅲ type pulmonary turberculosis ） and 97 healthy volunteers were enrolled in our study. The antigen specific IFN-r responses were evaluated by Elispot assay. Results The sensibility of Elispot in tuberculosis patients is 81.64% , and the specificity is 85.57%. Positive IFN-r response were detected in 61.64% tuberculous meningoencephalitis group, 86.13% in Ⅲ type pulmonary turberculosis group and 10.31% in healthy vvolunteers. There were statistical differences between groups （P 〈 0. 05 ）. Conclusion Antigen-specific IFN-r Elispot assay could be a favourable assistant method in the diagnosis of Tuberculosis. It has better value in Ⅲ type pulmonary tuberculosis than in tuberculous meningoencephalitis.

关 键 词：酶联免疫斑点技术(Elispot) 结核性脑膜脑炎 Ⅲ型肺结核 

分 类 号：R529.3[医药卫生—内科学] R521[医药卫生—临床医学]