成都地区淋病奈瑟菌质粒谱分析研究  

To Analyse and Study on Plasmid Profiles of Neisseria Gonorrhoeae Isolated in Chengdu

作　　者：向辉[1] 叶梅君[1] 刘衡川[1] 雍刚 李绍兴 

机构地区：[1]华西医科大学公共卫生学院医检教研室,成都610041 [2]四川省皮肤病性病防治研究所STD检测中心实验室

出　　处：《中国公共卫生》1999年第9期773-774,共2页Chinese Journal of Public Health

基　　金：四川省科委和四川省卫生厅基金

摘　　要：应用碱裂解加溶菌酶法对本地区１９９６ 年分离鉴定的１５５ 株标本进行了质粒抽提及质粒谱分型研究。结果表明：５种不同分子量的质粒分别被检出，其中分子量为５３４６ 和３９５ｋｂ 两种质粒检出率较高，分别为９６１３ ％ 和９０３２ ％ 。质粒谱型共１０ 种，主要以２１ｋｂ ＋ ５３ｋｂ ＋ ３９５ｋｂ 和２１ｋｂ ＋４２ｋｂ＋ ５３ｋｂ ＋ ７４ｋｂ ＋ ３９５ｋｂ 为主，占６３５８ ％ （９６／１５１） 。并将耐药谱型与质粒谱型进行了比较，具有相同质粒谱型的菌株，其耐药谱型可相同亦可不同。并地淋病奈瑟菌质粒抽提方法进行了探讨，摸索出了一种快速质粒检测的简便方法。To comprehend the state of plasmid carrying and plasmid profiles of Neissria gonorrhoeae isolated in chengdu area in 1996,155 strains gonococcus plasmids were extracted by alkali and lysozyme,meanwhile,the plasmid profiles were typed.The studied results showed that five kinds of different molecule weight plasmids were found,among them the presence of 5.3kb and 39.5kb plasmids were 96.13% and 90.32%,respectively,making up of ten kinds of plasmid profiles.The PP type of 2 1kb+5 3kb+39 5kb and 2 1kb+4 2kb+5 3kb+7 4kb+39 5kb were primary(63 58%).In comparison with antibiotic resistant profiles,the same PP type of strains there are different or identical antibiotic resistant profiles.The way of plasmid extracting was modified in this arthcle,and the modified assay was a simple plasmid check way which may be recommended as an alternative tradition way.

关 键 词：淋病奈瑟菌 质粒谱 分子流行病学 淋病 

分 类 号：R378.16[医药卫生—病原生物学] R759.2[医药卫生—基础医学]