补肾益气方对皮质酮致大鼠胸腺细胞凋亡相关基因表达的影响  

Effects of Bushenyiqi formula on the expressions of apoptosis related genes in Corticosterone damaged Rat thymocytes

作　　者：龚张斌[1] 徐品初[2] 金国琴[1] 

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]上海中医药大学附属曙光医院,上海201203

出　　处：《中华中医药杂志》2011年第4期672-675,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：胸国家自然基金(No.30873317);上海市教育委员会重点学科建设项目(No.J50301);上海教委基金(No.06CZ011)~~

摘　　要：目的:研究补肾益气方对皮质酮致大鼠胸腺细胞凋亡及Bcl-2、Bax、IL-2表达的影响。方法:1×10-5mol/L皮质酮处理乳鼠胸腺细胞制作病理细胞模型,分组:正常组;模型组;补肾益气组。Annexin V-PI双染色法结合FCM检测胸腺细胞凋亡;Real-time PCR和Western blotting法检测Bcl-2、Bax、IL-2的表达。结果:皮质酮诱导胸腺细胞凋亡率显著升高(P<0.01);bax表达显著升高,bcl-2/bax比值、IL-2表达降低(P<0.05)。与模型组比较,补肾益气组凋亡率降低(P<0.05);Bax mRNA表达降低,bcl-2/bax比值、IL-2表达升高(P<0.05)。结论:补肾益气方含药鼠血清能通过改善模型细胞bcl-2/bax的异常变化,抑制胸腺细胞过度凋亡,提高胸腺依赖性免疫功能。Objective：To investigate the molecular mechanism of Bushenyiqi Formula（BSYQ） on the apoptosis in Corticosterone damaged Rat thymocytes.Methods：Prepared the BSYQ contained rat serum.Thymocytes were divided into 3 groups：control group;model group;BSYQ group.The effect of apoptosis was measured by Annexin V-FITC/PI double staining and FCM.The expression of bcl-2,bax,Il-2 were measured by Real-time PCR and Western Blotting.Results：10-5 M corticosterone induced apoptosis of thymocytes,up-regulated the expression of bax,down-regulated the expression of IL-2,the ratio of bcl-2/bax was descended.Comparing with model group,BSYQ group showed lower expression of bax and higher expression of IL-2（P0.05）,the ratio of bcl-2/bax was elevated.Conclusions：BSYQ contained rat serum can inhibit the apoptosis of thymocytes induced by corticosterone.The possible mechanism of immuno-regulation effect of BSYQ is to regulate the expression of bax and bcl-2 in thymocytes.

关 键 词：胸腺细胞 细胞凋亡 免疫 Bcl-2 Bax IL-2 

分 类 号：R285.5[医药卫生—中药学]