2型糖尿病实验大鼠表征及其证候特征研究  

Research on characteristic and syndrome of type 2 diabetic rat models induced by streptozotocin

作　　者：潘秋[1] 赵慧辉[1] 陈建新[1] 韩静[1] 余俊达[1] 王伟[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2011年第4期683-685,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：教育部北京中医药大学自主选题项目(No.JYBZZ-XS008);科技部国际科技合作项目(No.2008DFA30610)~~

摘　　要：目的:探索高脂饮食加腹腔注射链脲佐菌素(streptozotocin,STZ)诱发2型糖尿病大鼠的表征及其证候特征。方法:按体质量将30只8周龄SD大鼠随机分为模型组和对照组各15只。模型组予以高脂饮食加腹腔注射STZ35mg/kg,对照组普通饮食加腹腔注射同等剂量柠檬酸-柠檬酸钠缓冲液。每周观察大鼠表征,检测24h饮食量、饮水量、尿量、体质量、体温和抓力,6周后处死检测血糖、胰岛素、血脂、血液流变学等指标。结果:与对照组相比,模型组形体消瘦、行动减少,毛色黄、耳鼻唇舌颜色淡白、大便黏滞松软、体质量低、抓力弱、血糖高、血液黏稠度高(P<0.05)。结论:高脂饮食加腹腔注射STZ诱发2型糖尿病大鼠第1-6周的表征基本稳定,课题组初步推测模型组大鼠证候特征为前期脾气亏虚证为主,后期痰瘀互阻证为主。Objective：To explore the characteristic and syndrome of diabetic rat models induced by high fat diet and streptozotocin.Methods：30 rats were randomly divided into two groups,15 in model group and 15 in control group.The rat models were fed with high-fat diet for 2 weeks plus intraperitoneal injection of STZ（35mg/kg）.The phisical signs were collected through 6 weeks,as well as the amount of food intake,water,urine,body weight,temperature and strength of grabbing.The fasting glucose,fasting insulin,blood-fat and hemorheology were tested after 6 weeks when rats were executed.Results：Compared with control group,the phisical signs such as slim shape,dull action,yellow fur,white ears,nose,slip and tongue,sticky and loose stool had statistical significance（P0.05）;blood glucose and hemorheology increased significantly（P0.05）;the body weight and strength of grabbing decreased significantly（P0.05）.Conclusion：The characteristic of diabetic rat models induced by high fat diet and STZ is stabile through the 1rd to 6th weeks.The characteristic may be diagnosed as the syndrome of spleen deficiency and intermingled phlegm and blood stasis.

关 键 词：表征 糖尿病 大鼠 脾气亏虚证 痰瘀互阻证 

分 类 号：R259[医药卫生—中西医结合]