异补骨脂素对H_2O_2诱导氧化损伤的人晶状体上皮细胞NF-κB表达的影响  

Effect of isopsoralen on expression of NF-κB in oxidativedamage of human lens epithelial cell induced by H_2O_2

作　　者：冯春燕[1,2] 黄秀榕[1] 祁明信[2] 唐宋文[1] 郭娜[2] 胡艳红[2] 

机构地区：[1]福建中医药大学,福州350003 [2]福建中医药大学附属第二人民医院,福州350003

出　　处：《中华中医药杂志》2011年第4期835-837,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省卫生厅青年科研课题(No.2009-1-30);福建省教育厅课题(No.JA10176)~~

摘　　要：目的:探讨具有雌激素活性的中药单体异补骨脂素(ISR)对过氧化氢(H2O2)诱导氧化损伤的人晶状体上皮细胞(HLEC)NF-κB表达的影响。方法:实验分正常组、H2O2组、雌二醇(E2)组、异补骨脂素(ISR)组、核因子-κB(NF-κB)抑制剂二硫代氨基甲酸吡咯烷(PDTC)组,流式细胞术(FCM)测定上述5组标本中HLEC的NF-κB的表达率。结果:以上各组,NF-κB的平均荧光表达率分别为:9.53%、39.87%、25.18%、23.23%、5.90%;正常HLEC能表达一定的NF-κB,H2O2组NF-κB的表达率明显增高,NF-κB抑制剂PDTC组的NF-κB的表达率明显降低,ISR组和E2组的NF-κB的表达率则介于以上两组之间。结论:H2O2可以诱导人晶状体上皮细胞NF-κB的活化表达,具有雌激素活性的中药单体ISR可以有效抑制NF-κB的活化表达,其抗HLEC氧化损伤作用可能是通过下调NF-κB表达实现的。Objective：To investigate the influence of isopsoralen（ISR） with estrogenic activity on expression of nuclear factor-kappa B（NF-κB） in HLEC afer oxidative damage induced by H2O2.Methods：The cultured HLEC were divided into 5 groups：control group,H2O2 group,β-Estradiol（E2） group,ISR group and PDTC（NF-κB inhibitor） group.The expression of NF-κB in HLEC was detected by flow cytometer（FCM）.Results：In the above groups,the mean fluorescence expression rate of NF-κB were：9.53%,39.87%,25.18%,23.23%,5.90%.The normal HLEC express NF-κB;The expression rate of NF-κB in H2O2 group was increased obviously compared with control group;The expression rate of NF-κB in PDTC（NF-κB inhibitor） group was decreased conspicuously compared with H2O2 group;The expression rates of NF-κB in ISR and E2 group were between H2O2 group and PDTC group.Conclusion：The activation of NF-κB in human lens epithelial cells could be induced by H2O2.ISR with estrogenic activity could inhibit the activation of NF-κB,the down-regulated NF-κB may be involved in the role of Antioxidant damage.

关 键 词：异补骨脂素 核因子-ΚB 人晶状体上皮细胞 氧化损伤 

分 类 号：R285[医药卫生—中药学]