黄芪甲苷联合树突状细胞瘤苗抗胃癌免疫的体外实验研究  

Research of antitumor immunity of gastric carcinoma by DC vaccine with AN in vitro

作　　者：杨璐[1] 沈洪[4] 王立新[5] 林琳[2] 周晓波[4] 吴静[4] 刘增巍[4] 徐艺[4] 赵崧[4] 葛超[4] 骆殊[3] 刘亚军[1] 

机构地区：[1]南京中医药大学第一临床医学院2008级硕士研究生,210046 [2]南京中医药大学2008级博士研究生,210046 [3]南京中医药大学基础医学院中医基础教研室,210046 [4]江苏省中医院消化内科,南京210029 [5]东南大学医学院病原生物学与免疫学系

出　　处：《国际中医中药杂志》2011年第4期301-304,共4页International Journal of Traditional Chinese Medicine

摘　　要：目的通过体外实验观察中药单体黄芪甲苷（astragaloside IV，AIV）联合树突状细胞（DC）瘤苗对机体免疫功能的调节作用。方法外周血单个核细胞体外诱导、培养，负载胃癌SGC7901冻融抗原，制备DC瘤苗，分别观察AⅣ联合DC瘤苗、DC瘤苗、AIV淋巴细胞增殖情况及其激活的效应细胞在体外杀伤胃癌细胞的活性。结果①AⅣ联合DC瘤苗（联合）组、DC瘤苗组T淋巴细胞增殖率显著高于AIV组、T细胞组（阴性对照组）（P=0．000），其中联合组促淋巴细胞增殖能力优于DC瘤苗组（S／R为1：5、1：10、1：50、1：100时，P分别为0．013、0．014、0．017、0．019）；AIV组T淋巴细胞增殖与T细胞组比较，差异无统计学意义（P=0．185）。②联合组及DC瘤苗组所活化的T细胞对胃癌SGC7901细胞的杀伤率高于AⅣ组、未活化T细胞组（P=0．000），联合组杀伤能力优于DC瘤苗组（E／T为5：1、10：1、20：1、50：1时，P分别为0．023、0．012、0．016、0．011）；而AⅣ组与未活化T细胞组几乎无杀伤作用（P=0．267）。结论AⅣ可通过DC瘤苗促进T淋巴细胞的增殖及杀伤肿瘤的活性，从而更有效地诱导抗胃癌特异性免疫应答。Objective Through experiments in vitro, we explored the role of Chinese medicine monomer--Astragaloside IV with DC vaccine in the body immunologic function. Methods Peripheral blood mononuclear cells （PBMCs） from healthy volunteers were isolated, cultured and generated in vitro, pulsed with tumor antigen from SGC7901 gastric carcinoma cell lysates, produced DC vaccine. Observe T-cell proliferation responses stimulated by DC vaccine with AIM group, DC vaccine group and AIV group respectively, and the anti-tumor effects on SGC7901 cells in vitro. Results OThe T-cell proliferation rate of DC vaccine with AIV group and DC vaccine group were significantly higher than AlVgroup and T-cell group （negative control group） （P=0.000） .The stimulating efficacy on T-cell proliferation of DC vaccine with AIVgroup was higher than that of DC vaccine group （S/R 1 ： 5, 1 ： 10, 1 ： 50, 1 ： 100, P=0.013, 0.014, 0.017, 0.019）. Compared with T-cell group, the T-cell proliferation rate of A1V group had no statistically significance （P= 0.185）. ~）The killing rate of effector cells actived by DC vaccine with AIV group and DC vaccine group against SGC7901 gastric carcinoma cells were higher than that of A1V group and T-cell group（P=0.000）.The killing power of DC vaccine with AIV group was stronger than that of DC vaccine （E/T 5 ： 1, 10 ： 1, 20 ： 1, 50 ： 1, P=0.023, 0.012, 0.016, 0.011）; while the group of AIVgroup and T-cell group cannot killing tumor cells. Both had no statistically significance （P=0.267）. Conclusion AIVcan stimulate T-cell proliferation and enhance the activity of killing tumor cells by DC, which induced specific antitumor response against stomach carcinoma cells effectively.

关 键 词：树突状细胞 黄芪甲苷 胃癌 抗肿瘤免疫 

分 类 号：R28[医药卫生—中药学]