青蒿琥酯对斯氏狸殖吸虫病大鼠肝脏组织病理改变及功能的影响  

Effects of artesunate on hepatic histopathologic change and function of rats infected with Pagumogonimus skrjabini

作　　者：李鹏华[1] 畅灵丽[1] 王光西[1] 李珍炼[1] 陈文碧[1] 张跃辉[1] 吴桐[1] 

机构地区：[1]泸州医学院病原生物学教研室,646000

出　　处：《国际医学寄生虫病杂志》2011年第2期69-73,共5页International JOurnal of Medical Parasitic Diseases

基　　金：基金项目：四川省教育厅科研基金（2003A062）

摘　　要：目的 对比观察青蒿琥酯和吡喹酮治疗大鼠斯氏狸殖吸虫病后，大鼠肝脏组织病理改变及功能变化。 方法 将36只清洁级Wistar大鼠随机分成6组，其中5组每只大鼠腹腔注射斯氏狸殖吸虫囊蚴25个，于感染后30 d开始用药治疗，治疗组给予青蒿琥酯50 mg/（kg＆#8226;d）、100 mg /（kg＆#8226;d）、150 mg /（kg＆#8226;d），连续给药7 d，吡喹酮组150 mg/kg隔日给药1次，共3次。对照组不做治疗，另设正常对照组。观察大鼠肝脏组织病理改变，摘眼球取血，测定肝功能。 结果 青蒿琥酯50 mg/（kg＆#8226;d）、100 mg/（kg＆#8226;d）与对照组比较，肝脏组织炎症改变减轻，肝脏未出现纤维化，青蒿琥酯50 mg/（kg＆#8226;d）、100 mg/（kg＆#8226;d）组白蛋白显著升高、球蛋白显著降低、谷丙转氨酶显著降低、白蛋白/球蛋白升高最为明显，与对照组相比差异有统计学意义（P＜0.05）。 结论 一定剂量的青蒿琥酯能减轻斯氏狸殖吸虫病大鼠肝脏的损伤，具有保护肝细胞、降酶作用。Objective To observe the effects of artesunate on hepatic histopathologic change and function of rats infected with Pagumogonimus skrjabini. Methods Thirty-six Wistar rats were randomly divided into 6 groups. Each rat of 5 groups was intra-abdominally injected with 25 metacercaria of P. skrjabini, 6 rats of one group were remained as normal control. Thirty days after the rats were infected with the metacerearia of P. skrjabini, the intragastric administration was performed in the experimental groups according to following sche- dule： artesunate 50 mg/（kg ~ d）, 100 mg/（kg ~ d） and 150 mg/（kg · d）, for consecutive 7 d, praziquantel 150 mg/kg q. o. d, for 3 times. Model control group was not treated. The rats were sacrificed for pathological examination with microscope after 30 d. Alanine aminotransferase （ ALT）, aspartate aminotransferase （ AST）, albumin （ALB） ,globulin（GLO） were detected in serum by automatic biochemical analyzer. Results Artesu- hate 50 mg/（ kg · d） and 100 rag/（ kg· d） treatment could reduce the liver inflammation of the rats infected with the metacercaria of P. skrjabini. There were significant differences between these two experimental groups and the control group （ P 〈 0.05 ）, on the levels of ALB ,GLO ,A/G,AST in serum. Conclusion Appropriate dose of artesunate may improve the hepatic function of rats infected with P. skrjabini.

关 键 词：斯氏狸殖吸虫 青蒿琥酯 肝功能 组织病理改变 

分 类 号：R532.2[医药卫生—内科学]