甲基强的松龙对大鼠脊髓损伤后星形胶质细胞的影响  

Effects of Methylprednisolone on Astrocyte after Spinal Cord Injury in Rats

作　　者：白旭东[1] 李洪鹏[1] 高杰[1] 巴方[2] 刘宁[3] 孙永新[3] 

机构地区：[1]中国医科大学基础医学院解剖教研室,辽宁沈阳市110001 [2]中国医科大学第二附属医院康复科,辽宁沈阳市110001 [3]中国医科大学第一附属医院康复科,辽宁沈阳市110001

出　　处：《中国康复理论与实践》2011年第3期219-222,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金资助项目(30872669);辽宁省教育厅资助项目(2008778)

摘　　要：目的观察大鼠脊髓损伤后甲基强的松龙(MP)对星形胶质细胞(AS)增殖和胶质纤维酸性蛋白(GFAP)表达的影响。方法雄性SPF级10周龄大鼠48只,制成脊髓右侧半横断损伤模型,分为MP组和对照组,每组24只。术后每4d进行1次行为学(BBB)评分。MP组通过尾静脉注射MP溶液,对照组同时注射等量生理盐水,两组大鼠分别在3d、7d、14d、28d处死,应用免疫组化技术观察GFAP的表达,并做灰度值测定和GFAP阳性细胞计数。结果与对照组相比,MP组在损伤后21d内行为学方面无显著性差异,在25d后MP组优于对照组。脊髓损伤后3d和7d,MP组活性化AS数目明显少于对照组,14d后无显著性差异;3d、7d、21d时,MP组胶质性瘢痕中GFAP表达量均明显低于对照组,28d时两组无显著性差异。结论急性脊髓损伤后大剂量MP冲击疗法能减少AS活化的数目,减少胶质性瘢痕的形成,并能促进后肢功能的恢复。Objective To investigate the effects of methylprednisolone （MP） on the proliferation and expression of glial fibrillary acidic protein （GFAP） in astrocytes after spinal cord injury in rats. MethodsThe spinal cords in T8~T10 in 48 male Wistar rats （10 weeks old） were exposed and the right site were semi-transected. 24 rats were administrated MP through tail vein as experiment group, and the other rats were injected equal volumes of normal saline as the control group. They were assessed with Basso, Beattie, and Bresnahan （BBB） score for ethological test every 4 d, and were sacrificed, perfused 3 d, 7 d, 14 d and 28 d after operation. The spinal cords were removed and postfixed, The expression of GFAP was observed with immunohistochemical staining and gray values determination. The GFAP positive cells were also counted. ResultsThere was no significant difference in BBB score between these groups until 21 d after operation; but the MP group rats showed significantly better functional recovery than the control group 25 d after operation. The number of positive astrocytes was less in the MP group than in the control group 3 and 7 d after operation, but there was no significant difference 14 d after operation. The GFAP expression in glial scar was significantly lower in the MP group than in the control in the first 21 d after operation, but no significant difference 28 d after operation. ConclusionThe large dose methylprednisolone administration can decrease the number of active AS, attenuate the formation of glial scars, and improve the functional recovery of the hind limbs after acute spinal cord injury.

关 键 词：脊髓损伤 甲基强的松龙 胶质纤维酸性蛋白 星形胶质细胞 大鼠 

分 类 号：R651.2[医药卫生—外科学]