三黄鸡临床多发性肿瘤相关的J亚群禽白血病病毒的分离鉴定及其病理学观察  

Isolation and identification of the subgroup J avian leukosis virus associated with clinical multiple tumours in Sanhuang layers and pathological observation

作　　者：齐新永[1] 张维谊[1] 鞠厚斌[1] 邓波[1] 周锦萍[1] 刘建[1] 王曲直[1] 

机构地区：[1]上海市动物疫病预防控制中心,上海201103

出　　处：《中国预防兽医学报》2011年第2期101-104,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：上海市重大动物疫病检测专项基金

摘　　要：为研究蛋鸡多发性肿瘤的病因,本实验对病鸡肿瘤组织进行病毒分离、培养及PCR检测,均扩增出针对J亚群禽白血病病毒(ALV-J)gp85基因序列的阳性条带;组织病理学观察显示发病鸡呈现髓细胞瘤、血管瘤以及纤维肉瘤等多发性肿瘤的病理变化;肿瘤细胞通过血液转移、浸润,在肝和脾组织形成局灶性或弥漫性肿瘤病灶。免疫组化染色显示肿瘤组织内,部分肿瘤细胞呈现阳性反应,表明只有部分肿瘤细胞存在ALV-J感染,而大部分肿瘤细胞检测结果呈阴性。这些病毒检测为阴性的肿瘤细胞可能是正常细胞转化为肿瘤细胞大量克隆化增殖的结果。To understand the causes of multiple tumors,clinic tumor samples were collected from affected Sanhuang layers to isolate virus in chick embryo fibroblast（CEF）.Both of the tusse samples and virus isolation in CEFs were positive,detected by RT-PCR with specific primers for gp85 gene of subgroup J avian leukosis virus（ALV-J）.Histopathologic examination of tissue samples showed that the multiple tumours were myelocytoma,hemangioma or fibrosarcoma due to tumor cells transferred and infiltrated by blood to forme focal or diffusing neoplastic lesions in liver and spleen.Through immunohistological localization,some tumor cells showed positive which indicated only partial tumor cells were infected by ALV-J,while most tumor cells showed negative.indicating most tumor cells were proliferation resulting from normal transferred cells.

关 键 词：J亚群禽白血病病毒 多发性肿瘤 组织病理学 免疫组化 

分 类 号：S852.65[农业科学—基础兽医学]