应用基因芯片技术筛选皮肤光老化相关基因  

Screening of genes associated with skin photoaging by microarray analysis

作　　者：颜薇[1] 张利利[2] 刘少辉[3] 石兵兵[1] 赵振民[1] 王敦梅[2] 尹宁北[1] 

机构地区：[1]中国医学科学院北京协和医学院整形外科医院,北京100144 [2]中国科学院基因组所 [3]中国医学科学院北京协和医学院机关医务室

出　　处：《中华皮肤科杂志》2011年第2期130-133,共4页Chinese Journal of Dermatology

摘　　要：目的 探讨光老化皮肤与正常皮肤的基因表达差异,在转录组水平上探讨光老化分子生物学机制.方法 选取2007-2008年入院面部光老化患者6例,收集患者全颜面除皱术中切除的耳前（光曝露）、耳后（光保护）全层皮肤,进行全基因组表达芯片分析,筛选差异基因并进行功能聚类分析.结果 1例因数据差异明显被排除,对其他5例数据分组分析共筛选差异表达基因172个,上调99个,下调73个.筛选出的差异基因涉及细胞黏附及受体调节、信号转导、代谢等多个生物学过程.结论 光老化皮肤与正常皮肤之间存在多处基因表达差异.除皱史可能与皮肤光老化基因差异表达程度存在一定联系.Objective To detect differentially expressed genes between human normal skin and photoaging skin, and to investigate the molecular and biological mechanisms of human skin photoaging at transcriptional level. Methods Full-thickness skin specimens were obtained during full-face rhytidectomy from sun-exposed （anterior ear skin） and sun-protected （retroauricular skin） sites of 6 patients with facial photoaging from 2007 to 2008. Genomic microarray analysis was conducted to identify differentially expressed genes between the two groups of specimens followed by gene-cluster analysis. Results The normalization of microarray data showed that the number of differentially expressed genes was 2163 between skin samples from sun-exposed and sun-protected sites in one patient, significantly higher than that in the other 5 patients （less than 200）;therefore, the data from the patient with 2163 differentially expressed genes were excluded from further analysis.Totally, 172 differentially expressed genes were identified with Beadarray chip, including 99 up-regulated genes and 73 down-regulated genes. Based on Genebank research, 118 functionally classified genes werefound, which were associated with a series of biological processes, including cell adhesion, receptor regulation,signal transduction, metabolism, and so on. Conclusions There are a lot of differentially expressed genes between human photoaging skin and normal skin. Rhytidectomy may be associated with the differential expression of skin photoaging-related genes.

关 键 词：皮肤衰老 寡核苷酸序列分析 差异表达基因 

分 类 号：R622[医药卫生—整形外科]